A flow cytometric granularity assay for the quantification of infectious virus
| Autor: | Patrick Farrell, Julia Manalil, Christina Notte, Steve George, Arno Zeiser, Marc G. Aucoin, Megan Logan, Craig Kearse |
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| Rok vydání: | 2018 |
| Předmět: |
viruses
Herpesvirus 2 Human 030231 tropical medicine Canarypox virus Viral Plaque Assay Biology medicine.disease_cause Virus Flow cytometry law.invention Cell Line 03 medical and health sciences 0302 clinical medicine law Chlorocebus aethiops medicine Animals 030212 general & internal medicine Vero Cells Virus quantification General Veterinary General Immunology and Microbiology medicine.diagnostic_test Public Health Environmental and Occupational Health Viral Load Flow Cytometry Virology Molecular biology Titer Infectious Diseases Herpes simplex virus Virus Diseases Recombinant DNA Molecular Medicine Granularity |
| Zdroj: | Vaccine. 37(47) |
| ISSN: | 1873-2518 |
| Popis: | A flow cytometry-based assay was developed to assess the infective titer of two recombinant viruses: a recombinant herpes simplex type 2 (rHSV-2) and a recombinant canary pox (rALVAC.gfp). This method uses granularity of infected Vero and QT-35 cells, respectively, and correlates this to the infectious titer of virus samples. The percent of the cell populations with a high level of granularity could accurately be correlated to viral titers obtained through a traditional plaque assay, with R2 values greater than 0.8 using a semi-logarithmic scale. This approach offers a rapid, high-throughput method for infectious virus titration with similar accuracy to a traditional plaque assay. |
| Databáze: | OpenAIRE |
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