Importance of particle size of oligomannose-coated liposomes for induction of Th1 immunity
| Autor: | Emi Onohara, Naoya Kojima, Yuko Matsuoka, Yasuhiro Kuroda |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Cytochalasin D
1 2-Dipalmitoylphosphatidylcholine Phagocytosis medicine.medical_treatment Immunology Spleen Interleukin-12 Subunit p35 Interferon-gamma Mice Immune system Antigen medicine Immunology and Allergy Animals Secretion Antigens Particle Size Pharmacology Liposome MHC class II Phagocytes biology Chemistry Histocompatibility Antigens Class II Th1 Cells medicine.anatomical_structure Cytokine Immune System Immunoglobulin G Liposomes Peritoneal Absorption biology.protein Female B7-2 Antigen Mannose |
| Zdroj: | International immunopharmacology. 99 |
| ISSN: | 1878-1705 |
| Popis: | Oligomannose-coated liposomes (OMLs) comprised of dipalmitoylphosphatidylcholine, cholesterol and Man3-DPPE at a molar ratio of 1:1:0.1 and particle diameters of about 1000 nm can induce liposome-encased antigen-specific strong Th1 immunity. In this study, we evaluated the effect of particle sizes of OMLs on induction of Th1 immune responses in mice. Spleen cells obtained from mice immunized with antigen-encapsulating OMLs with 1000- and 800-nm diameters secreted remarkably high levels of IFN-γ upon in vitro stimulation. In addition, sera of mice that received these OMLs had significantly higher titers of antigen-specific IgG2a than those of IgG1, which are commonly associated with Th1 responses. In contrast, treatment with antigen-encapsulating OMLs with 400- and 200-nm diameters failed to induce IFN-γ secretion from spleen cells, although these OMLs did elicit elevation of antigen-specific IgGs. In addition, the titers of serum antigen-specific IgG2a were the same as those of IgG1 in mice that received 400-nm OMLs. Resident peritoneal mononuclear phagocytes (MNPs) treated with OMLs of diameter ≥ 600 nm secreted IL-12, which is essential for induction of Th1 immune responses, while those treated with OMLs of ≤ 400 nm failed to produce this cytokine. However, 400-nm OMLs did induce enhanced expression of MHC class II and costimulatory molecules on MNPs, similarly to OMLs of ≥ 600 nm. Taken together, these results strongly indicate that OMLs of diameter ≥ 600 nm are required to induce Th1 immune responses against OML-encased antigens, although OMLs of diameter ≤ 400 nm can activate MNPs. |
| Databáze: | OpenAIRE |
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