Role of concentration and size of intracellular macromolecules in cell volume regulation
| Autor: | C. Pena-Rasgado, R. Buechler, R. Lajvardi, L. Trais, H. Chang, J. C. Summers, David Hergan, Hector Rasgado-Flores |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Sucrose
Molar concentration Macromolecular Substances Physiology Chemistry Cells Muscles Osmolar Concentration Thoracica Cell Povidone Intracellular Membranes Cell Biology Anatomy medicine.anatomical_structure Volume (thermodynamics) Sense (molecular biology) medicine Biophysics Animals Myocyte Isotonic Solutions Macromolecular crowding Intracellular Macromolecule |
| Zdroj: | American Journal of Physiology-Cell Physiology. 273:C360-C370 |
| ISSN: | 1522-1563 0363-6143 |
| DOI: | 10.1152/ajpcell.1997.273.2.c360 |
| Popis: | To gain insight into the mechanism(s) by which cells sense volume changes, specific predictions of the macromolecular crowding theory (A. P. Minton. In: Cellular and Molecular Physiology of Cell Volume Regulation, edited by K. Strange. Boca Raton, FL: CRC, 1994, p. 181-190. A. P. Minton, C. C. Colclasure, and J. C. Parker. Proc. Natl. Acad. Sci. USA 89: 10504-10506, 1992) were tested on the volume of internally perfused barnacle muscle cells. This preparation was chosen because it allows assessment of the effect on cell volume of changes in the intracellular macromolecular concentration and size while maintaining constant the ionic strength, membrane stretch, and osmolality. The predictions tested were that isotonic replacement of large macromolecules by smaller ones should induce volume decreases proportional to the initial macromolecular concentration and size as well as to the magnitude of the concentration reduction. The experimental results were consistent with these predictions: isotonic replacement of proteins or polymers with sucrose induced volume reductions, but this effect was only observed when the replacement was > or = 25% and the particular macromolecule had an average molecular mass of < or = 20 kDa and a concentration of at least 18 mg/ml. Volume reduction was effected by a mechanism identical with that of hypotonicity-induced regulatory volume decrease, namely, activation of verapamil-sensitive Ca2+ channels. |
| Databáze: | OpenAIRE |
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