Selenium supplementation induces mitochondrial biogenesis in trophoblasts
| Autor: | Elham Alizadeh Pasdar, Anthony V. Perkins, Jiri Neuzil, Jessica J. Vanderlelie, Olivia J. Holland, Lan-Feng Dong, Alisha Khera |
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| Rok vydání: | 2015 |
| Předmět: |
Antioxidant
medicine.medical_treatment Placenta NF-E2-Related Factor 1 chemistry.chemical_element Biology Mitochondrion medicine.disease_cause Cell Line Selenium Pregnancy medicine Humans Selenoproteins chemistry.chemical_classification Reactive oxygen species Organelle Biogenesis food and beverages Obstetrics and Gynecology Trophoblast Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha Cell biology Mitochondria Trophoblasts Up-Regulation Blot DNA-Binding Proteins medicine.anatomical_structure Reproductive Medicine Biochemistry Mitochondrial biogenesis chemistry embryonic structures Female Reactive Oxygen Species Oxidative stress Developmental Biology Transcription Factors |
| Zdroj: | Placenta. 36(8) |
| ISSN: | 1532-3102 |
| Popis: | Introduction Placental oxidative stress has been implicated in pregnancy complications and previous work has shown that selenium can protect trophoblast mitochondria from oxidative stress. This report examines mitochondrial function and content in trophoblasts supplemented with selenium. Methods Swan-71, JEG-3 and BeWo cells and placental tissue were incubated with sodium selenite or selenomethionine. Mitochondrial function was examined in a respirometer. Mitochondrial content was determined using RT-PCR. The levels of the mitochondrial biogenesis markers selenoprotein H, PGC-1α and NRF-1 was examined by western blotting. Results Mitochondrial respiration was significantly enhanced post selenium supplementation in cells and tissues. Selenium supplementation increased mitochondrial content and up-regulated mitochondrial biogenesis mediators in cells. Discussion These results emphasise the importance of selenium in mitochondrial regeneration in trophoblasts. |
| Databáze: | OpenAIRE |
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