Study of serotonin interactions with brush border membrane of rabbit jejunum enterocytes
| Autor: | Angeles Lahuerta, Ana I. Alcalde, Victor Sorribas, M. Jesús Rodríguez-Yoldi |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Male
Serotonin Indoles Brush border Enterocyte Biology Tritium Binding Competitive medicine Animals Ergolines 5-HT receptor Pharmacology Sulfonamides Dose-Response Relationship Drug Microvilli Vesicle Cell Membrane Biological Transport Membrane transport Ondansetron Dissociation constant Kinetics Membrane medicine.anatomical_structure Enterocytes Jejunum Biochemistry Competitive antagonist Biophysics Rabbits Serotonin Antagonists |
| Zdroj: | European journal of pharmacology. 403(1-2) |
| ISSN: | 0014-2999 |
| Popis: | Recent studies have demonstrated that serotonin (5-hydroxytryptamine, 5-HT) may interact with either specific receptors or with a specific transporter that takes up 5-HT in the gastrointestinal tract. The purpose of the present work was to study the 5-HT interactions with brush border membrane from rabbit jejunum enterocytes. The results obtained showed that 5-HT did not seem to be transported by any specific system of transport in brush border membrane vesicles. Nevertheless, [3H]5-HT seemed to bind specifically to this membrane. The kinetic analysis indicated a saturable and dissociable specific binding with a dissociation constant KD=14×10−9 M. The saturation studies with [3H]5-HT indicated the presence of one specific site in the brush border membrane. The results of displacement of [3H]5-HT specific binding from the brush border membrane showed that both unlabelled 5-HT and unlabelled GR113080 ([1-[(2-methyl sulphonyl) amino] ethyl-4-piperidinyl] methyl-1-methyl-1H-indole-3-carboxylate), a specific competitive antagonist of 5-HT4 receptors, inhibited the specific binding of [3H]5-HT to this membrane. |
| Databáze: | OpenAIRE |
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