Long non-coding RNA HCAR promotes endochondral bone repair by upregulating VEGF and MMP13 in hypertrophic chondrocyte through sponging miR-15b-5p
| Autor: | Chunrong Zhao, Qijie Dai, Zhansong Tian, Ce Dou, Rui Dong, Fei Kang, Jingjin Dai, Zhen Cao, Yun Bai, Jiulin Tan, Shiwu Dong, Jianmei Li, Xiaoshan Gong, Chuan Liu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Medicine (General) Matrix metallopeptidase 13 Enchondral bone repair Bone healing QH426-470 Biochemistry Bone marrow mesenchymal stem cells 03 medical and health sciences chemistry.chemical_compound R5-920 0302 clinical medicine Genetics medicine Molecular Biology Endochondral ossification Genetics (clinical) Gene knockdown Long non coding RNA Chemistry Cartilage Cell Biology Chondrocyte Long non-coding RNA Cell biology Vascular endothelial growth factor Vascular endothelial growth factor A 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Bone repair |
| Zdroj: | Genes and Diseases, Vol 9, Iss 2, Pp 456-465 (2022) |
| ISSN: | 2352-3042 |
| Popis: | Endochondral bone formation is an important route for bone repair. Although emerging evidence has revealed the functions of long non-coding RNAs (lncRNAs) in bone and cartilage development, the effect of lncRNAs in endochondral bone repair is still largely unknown. Here, we identified a lncRNA, named Hypertrophic Chondrocyte Angiogenesis-related lncRNA (HCAR), and proved it to promote the endochondral bone repair by upregulating the expression of matrix metallopeptidase 13 (Mmp13) and vascular endothelial growth factor α (Vegfa) in hypertrophic chondrocytes. Lnc-HCAR knockdown in hypertrophic chondrocytes restrained the cartilage matrix remodeling and decrease the CD31hiEmcnhi vessels number in a bone repair model. Mechanistically, we proved that lnc-HCAR was mainly enriched in the cytoplasm using fluorescence in situ hybridization (FISH) assay, and it acted as a molecular sponge for miR-15b-5p. Further, in hypertrophic chondrocytes, lnc-HCAR competitively bound to miR-15b-5p to increase Vegfa and Mmp13 expression. Our results proved that lncRNA is deeply involved in endochondral bone repair, which will provide a new theoretical basis for future strategies for promoting fracture healing. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|