Group A Streptococcus establishes pharynx infection by degrading the deoxyribonucleic acid of neutrophil extracellular traps
| Autor: | Bo Zhu, Hitomi Mimuro, Yoshitoshi Ogura, Tokuju Okano, Miho Kurosawa, Mototsugu Tanaka, Masaomi Nangaku, Ryo Kinoshita-Daitoku, Kotaro Kiga, Tetsuya Hayashi, Tamako Iida, Koshu Okubo, Takahito Sanada, Ichiro Nakagawa, Toshihiko Suzuki, Junichi Hirahashi, Chihiro Aikawa |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Extracellular Traps Bacterial immune evasion Neutrophils Streptococcus pyogenes Science 030106 microbiology lcsh:Medicine Mucous membrane of nose Apoptosis medicine.disease_cause Real-Time Polymerase Chain Reaction Article Microbiology 03 medical and health sciences Mice Streptococcal Infections medicine Extracellular Animals Humans lcsh:Science Multidisciplinary Deoxyribonucleases Chemistry Streptococcus Macrophages lcsh:R Deoxyribonuclease Pharyngitis Neutrophil extracellular traps DNA Mice Inbred C57BL Disease Models Animal 030104 developmental biology Mutation Medicine Pharynx Nasal administration lcsh:Q |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-11 (2020) |
| ISSN: | 2045-2322 |
| Popis: | Group A Streptococcus (GAS) secretes deoxyribonucleases and evades neutrophil extracellular killing by degrading neutrophil extracellular traps (NETs). However, limited information is currently available on the interaction between GAS and NETs in the pathogenicity of GAS pharyngitis. In this study, we modified a mouse model of GAS pharyngitis and revealed an essential role for DNase in this model. After intranasal infection, the nasal mucosa was markedly damaged near the nasal cavity, at which GAS was surrounded by neutrophils. When neutrophils were depleted from mice, GAS colonization and damage to the nasal mucosa were significantly decreased. Furthermore, mice infected with deoxyribonuclease knockout GAS mutants (∆spd, ∆endA, and ∆sdaD2) survived significantly better than those infected with wild-type GAS. In addition, the supernatants of digested NETs enhanced GAS-induced cell death in vitro. Collectively, these results indicate that NET degradation products may contribute to the establishment of pharyngeal infection caused by GAS. |
| Databáze: | OpenAIRE |
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