Differential Effects of Colchicine on Cardiac Cell Viability in an in vitro Model Simulating Myocardial Infarction
| Autor: | Michal Entin-Meer, Jeremy Ben-Shoshan, Gilad Margolis, Einat Hertzberg-Bigelman, Ran Levy, Gad Keren |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Cell Survival Myocardial Infarction Apoptosis 030204 cardiovascular system & hematology Cell Line 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Internal medicine Medicine Colchicine Myocyte Animals Pharmacology (medical) Myocytes Cardiac Viability assay Myocardial infarction business.industry Tumor Necrosis Factor-alpha medicine.disease Tubulin Modulators Rats Clinical trial 030104 developmental biology chemistry Heart failure Cardiology Tumor necrosis factor alpha Cardiology and Cardiovascular Medicine business Myoblasts Cardiac |
| Zdroj: | Cardiology. 134(1) |
| ISSN: | 1421-9751 |
| Popis: | Objectives: We aimed to examine the effects of colchicine, currently in clinical trials for acute myocardial infarction (AMI), on the viability of cardiac cells using a cell line model of AMI. Methods: HL-1, a murine cardiomyocyte cell line, and H9C2, a rat cardiomyoblast cell line, were incubated with TNFα or sera derived from rats that underwent AMI or sham operation followed by addition of colchicine. In another experiment, HL-1/H9C2 cells were exposed to anoxia with or without subsequent addition of colchicine. Cell morphology and viability were assessed by light microscopy, flow cytometry and Western blot analyses for apoptotic markers. Results: Cellular viability was similar in both sera; however, exposing both cell lines to anoxia reduced their viability. Adding colchicine to anoxic H9C2, but not to anoxic HL-1, further increased their mortality, at least in part via enhanced apoptosis. Under any condition, colchicine induced detachment of H9C2 cells from their culture plates. This phenomenon did not apply to HL-1 cells. Conclusions: Colchicine enhanced cardiomyoblast mortality under in vitro conditions mimicking AMI and reduced their adherence capability. HL-1 was not affected by colchicine; nevertheless, no salvage effect was observed. We thus conclude that colchicine may not inhibit myocardial apoptosis following AMI. |
| Databáze: | OpenAIRE |
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