A single residue change in the product of the chrysanthemum gene TPL1-2 leads to a failure in its repression of flowering
| Autor: | Zixin Zhang, Xiaojuan Xing, Hua Cheng, Qian Hu, Jiafu Jiang, Fadi Chen, Weixin Liu, Yanan Liu, Peilei Cheng, Sumei Chen |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Time Factors Chrysanthemum Transgene Mutant Arabidopsis Mutagenesis (molecular biology technique) Flowers Plant Science Biology Genes Plant Real-Time Polymerase Chain Reaction medicine.disease_cause 01 natural sciences 03 medical and health sciences Gene Expression Regulation Plant Two-Hybrid System Techniques Gene expression Genetics medicine Arabidopsis thaliana Gene Plant Proteins Mutation Gene Expression Profiling fungi Wild type food and beverages Sequence Analysis DNA General Medicine Plants Genetically Modified biology.organism_classification Cell biology 030104 developmental biology Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Plant Science. 285:165-174 |
| ISSN: | 0168-9452 |
| DOI: | 10.1016/j.plantsci.2019.04.027 |
| Popis: | The TPL/TPR co-repressor is involved in many plant signaling pathways, including those regulating the switch from vegetative to reproductive growth. Here, a TPL homolog (TPL 1-2) was isolated from chrysanthemum. Its product was found to be deposited in the nucleus. The abundance of TPL1-2 transcript varied across the plant, with its highest level being recorded in the stem apex, and its lowest in the root and stem. In the leaf, the abundance of TPL1-2 transcript was highest at dusk in plants exposed to long days, and at dawn in those exposed to short days. Site-directed mutagenesis was used to induce an N176H mutation in TPL1-2. The constitutive expression in Arabidopsis thaliana of the wild type and the mutated alleles of TPL1-2 had a contrasting effect on flowering time, with the mutant transgene expressors flowering later than the wild type transgene expressors. The flowering-related genes FT, TSF, FUL and AP1 were all more strongly transcribed in the mutant transgene expressors than in the wild type transgene expressors. |
| Databáze: | OpenAIRE |
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