Fluorescence Modulation of Green Fluorescent Protein Using Fluorinated Unnatural Amino Acids
| Autor: | Hong-Anh Tran, Brent L Russell, Stephanie Gianturco, Douglas D. Young, Elizabeth J. Harbron, Konark Bhasin, Megha A. Vipani, Jordan K. Villa |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
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Molecular 0301 basic medicine green fluorescent protein Fluorophore Halogenation Green Fluorescent Proteins Mutant Molecular Conformation Pharmaceutical Science Mutagenesis (molecular biology technique) Biosensing Techniques 01 natural sciences Fluorescence Article Analytical Chemistry Green fluorescent protein lcsh:QD241-441 03 medical and health sciences Bimolecular fluorescence complementation chemistry.chemical_compound fluorotyrosine lcsh:Organic chemistry Drug Discovery Amino Acids Physical and Theoretical Chemistry Fluorescent Dyes chemistry.chemical_classification 010405 organic chemistry Chemistry Organic Chemistry Wild type biosensors 0104 chemical sciences Amino acid Spectrometry Fluorescence 030104 developmental biology Biochemistry Chemistry (miscellaneous) Mutation Molecular Medicine unnatural amino acids |
| Zdroj: | Molecules, Vol 22, Iss 7, p 1194 (2017) Molecules (Basel, Switzerland) |
| ISSN: | 1420-3049 |
| Popis: | The ability to modulate protein function through minimal perturbations to amino acid structure represents an ideal mechanism to engineer optimized proteins. Due to the novel spectroscopic properties of green fluorescent protein, it has found widespread application as a reporter protein throughout the fields of biology and chemistry. Using site-specific amino acid mutagenesis, we have incorporated various fluorotyrosine residues directly into the fluorophore of the protein, altering the fluorescence and shifting the pKa of the phenolic proton associated with the fluorophore. Relative to wild type GFP, the fluorescence spectrum of the protein is altered with each additional fluorine atom, and the mutant GFPs have the potential to be employed as pH sensors due to the altered electronic properties of the fluorine atoms. |
| Databáze: | OpenAIRE |
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