Lymphocyte apoptosis co-cultured with Borrelia burgdorferi
| Autor: | Presani Gianni, Prodan Mario, Perticarari Sandra, Cinco Marina, Granzotto Marilena, Murgia Rossella |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Lipoproteins
CD3 Cell Culture Techniques Apoptosis Microbiology Peripheral blood mononuclear cell CD19 Flow cytometry chemistry.chemical_compound Annexin medicine Humans Lymphocytes fas Receptor Propidium iodide Annexin A5 Borrelia burgdorferi Cells Cultured Antigens Bacterial biology medicine.diagnostic_test Antibodies Monoclonal Flow Cytometry biology.organism_classification Lymphocyte Subsets Interleukin-10 Bacterial vaccine Infectious Diseases chemistry Antigens Surface Bacterial Vaccines Leukocytes Mononuclear biology.protein Bacterial Outer Membrane Proteins Propidium |
| Zdroj: | Microbial Pathogenesis. 35:139-145 |
| ISSN: | 0882-4010 |
| DOI: | 10.1016/s0882-4010(03)00096-2 |
| Popis: | Borrelia burgdorferi is the causative agent of Lyme disease. We investigated whether the in vitro co-cultivation of lymphocytes with spirochetes would induce apoptosis in human lymphocytes. Peripheral blood mononuclear cell were mixed with various ratio of cell/spirochetes (1:10, 1:20, 1:50, 1:100) and incubated in a humified atmosphere of 5% CO(2) at 37 degrees C. Apoptosis was determined at 0, 4, 24 h by Annexin V binding assay and propidium iodide staining, and by CD95 Apo-1 expression. Analysis was performed by multiparametric flow cytometry on CD3, CD4, CD8, CD19 subset of lymphocytes. The binding of Annexin V increased at 24 h in T lymphocytes infected by living spirochetes at ratio 1:50; similar results were obtained with inactivated or sonicated spirochetes and lipidated OspC. The rate of Annexin V binding and pattern of CD95 over-expression were different in CD3, CD4, CD8 and CD19 subset; interleukine-10 (IL-10) was measured in supernatants of cultures after treatment with Borrelia preparations and with OspA and OspC, lipidated or not. Our data suggest that spirochetes were able to induce apoptosis on lymphocytes; the phenomenon appears associated with number of spirochetes, incubation time and the release of IL-10 in co-cultures. Moreover apoptosis was probably Fas-mediated and the cells involved were prevalently CD4. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect