Regionalized Twist1 activity in the forelimb bud drives the morphogenesis of the proximal and preaxial skeleton
| Autor: | Angelyn C. C. Hor, Vanessa Jones, David A.F. Loebel, Patrick P.L. Tam, Richard R. Behringer, You-Tzung Chen, Heidi Bildsoe |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Apical ectodermal ridge
Mesoderm animal structures Genotype Body Patterning Limb bud formation Fluorescent Antibody Technique Apoptosis Biology Mice 03 medical and health sciences Limb bud 0302 clinical medicine Forelimb Basic Helix-Loop-Helix Transcription Factors In Situ Nick-End Labeling Morphogenesis medicine Animals Limb development Hedgehog Proteins Molecular Biology Crosses Genetic In Situ Hybridization DNA Primers 030304 developmental biology 0303 health sciences Twist-Related Protein 1 Nuclear Proteins Cell Biology Anatomy beta-Galactosidase Fibroblast Growth Factors Mice Inbred C57BL body regions medicine.anatomical_structure Zone of polarizing activity Bone Morphogenetic Proteins Gene Deletion 030217 neurology & neurosurgery Signal Transduction Developmental Biology |
| Zdroj: | Developmental Biology. 362:132-140 |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/j.ydbio.2011.11.020 |
| Popis: | Development of the mouse forelimb bud depends on normal Twist1 activity. Global loss of Twist1 function before limb bud formation stops limb development and loss of Twist1 throughout the mesenchyme after limb bud initiation leads to polydactyly, the ulnarization or loss of the radius and malformations and reductions of the shoulder girdle. Here we show that conditional deletion of Twist1 by Mesp1-Cre in the mesoderm that migrates into the anterior-proximal part of the forelimb bud results in the development of supernumerary digits and carpals, the acquisition of ulna-like characteristics by the radius and malformations of the humerus and scapula. The mirror-like duplications and posteriorization of pre-axial tissues are preceded by disruptions to anterior-posterior Shh, Bmp and Fgf signaling gradients and dysregulation of transcription factors that regulate anterior-posterior limb patterning. |
| Databáze: | OpenAIRE |
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