Evaluation of tissue factor bearing microparticles as biomarkers in allogeneic stem-cell transplantation
| Autor: | Roswith Eisert, Christiane De Rop, Arnold Ganser, Arne Trummer, Stefanie Buchholz, Michael B. Stadler |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
Blood Platelets Male Mucositis medicine.medical_specialty Adolescent Graft vs Host Disease Gastroenterology Flow cytometry Thromboplastin Tissue factor Young Adult Cell-Derived Microparticles Predictive Value of Tests Recurrence Risk Factors Internal medicine Thromboembolism medicine Humans Transplantation Homologous Platelet Prospective Studies Prospective cohort study Aged Transplantation medicine.diagnostic_test business.industry Incidence Hazard ratio Middle Aged Flow Cytometry Hematologic Diseases Predictive value of tests Immunology Female Endothelium Vascular Stem cell business Biomarkers Stem Cell Transplantation |
| Zdroj: | Transplantation. 92(3) |
| ISSN: | 1534-6080 |
| Popis: | Background There is compelling evidence that blood-borne tissue factor that is predominantly found on circulating microparticles (MPs) plays an important role in both, cancer biology and organ and stem-cell transplantation (SCT). Therefore, we hypothesized that numbers of tissue factor bearing MPs might be associated with complications and outcome in allogeneic SCT (allo-SCT). Materials and methods In a prospective study, we enumerated total, platelet, endothelial, and tissue factor bearing MPs in plasma samples obtained from up to 60 patients with hematologic diseases at different time-points during the course of allo-SCT by flow cytometry. Patient- and transplant-related risk factors were included in statistical analysis. Results Mean follow-up time was 968 days (0-1981 days). Thirty-four (56.7%) patients died, 17 due to transplant-related mortality (28.3%). High numbers of tissue factor positive MPs more than 500/μL before conditioning were predictive for shorter overall survival (P=0.017, hazard ratio=4.5) in multivariate analysis. This was mainly caused by an increase in transplant-related mortality (P=0.010, hazard ratio=11.0) with cumulative incidences at 1 year of 68.8% compared with patients with lower values (20.1%; P=0.002). Conclusions Tissue factor bearing MPs might be useful biomarkers for risk stratification in allo-SCT patients and further studies should investigate their origin, functional properties, and optimal cut-off values. |
| Databáze: | OpenAIRE |
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