Gene transfer from mitochondrion to nucleus: novel mechanisms for gene activation from Cox2
| Autor: | Keith L. Adams, A. Harvey Millar, Daniel O. Daley, Jeffrey D. Palmer, Svenja Qualmann, Rachel Clifton, James Whelan, Elke Pratje |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Transcriptional Activation
Protein subunit Blotting Western Molecular Sequence Data Plant Science Mitochondrion Biology Models Biological Electron Transport Complex IV Species Specificity Gene Expression Regulation Plant Genetics Cytochrome c oxidase Amino Acid Sequence Gene Plant Proteins Cell Nucleus chemistry.chemical_classification Regulation of gene expression Genetic transfer Intron food and beverages Biological Transport Cell Biology Mitochondria Amino acid Biochemistry chemistry biology.protein Soybeans Protein Processing Post-Translational Gene Deletion |
| Zdroj: | The Plant Journal. 30:11-21 |
| ISSN: | 1365-313X 0960-7412 |
| DOI: | 10.1046/j.1365-313x.2002.01263.x |
| Popis: | The evolutionarily recent transfer of the gene for cytochrome c oxidase subunit 2 (cox2) from the mitochondrion to the nucleus in legumes is shown to have involved novel gene-activation steps. The acquired mitochondrial targeting presequence is bordered by two introns. Characterization of the import of soybean Cox2 indicates that the presequence is cleaved in a three-step process which is independent of assembly. The final processing step takes place only in the mitochondria of legume species, and not in several non-legume plants. The unusually long presequence of 136 amino acids consists of three regions: the first 20 amino acids are required for mitochondrial targeting and can be replaced by another presequence; the central portion of the presequence is required for efficient import of the Cox2 protein into mitochondria; and the last 12 amino acids, derived from the mitochondrially encoded protein, are required for correct maturation of the imported protein. The acquisition of a unique presequence, and the capacity for legume mitochondria to remove this presequence post-import, are considered to be essential adaptations for targeting of Cox2 to the mitochondrion and therefore activation of the transferred gene in the nucleus. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text