AP-1 and clathrin are essential for secretory granule biogenesis in Drosophila
| Autor: | Miluska Jauregui, Jason Burgess, Helmut Krämer, Julie A. Brill, Peter A. Leventis, Sylvie Lallet, Henry C. Chang, Janet Rollins, Julie Tan, Roland Le Borgne, Gabrielle L. Boulianne |
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| Přispěvatelé: | Department of Molecular Genetics [Toronto], University of Toronto, Program in Developmental and Stem Cell Biology, Hospital for Sick Children, Division of Natural Sciences, College of Mount Saint Vincent, Institut de Génétique et Développement de Rennes (IGDR), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), Department of Biological Sciences [West Lafayette], Purdue University [West Lafayette], Department of Neuroscience, University of Texas Southwestern Medical Center [Dallas], Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), De Villemeur, Hervé |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
MESH: Secretory Vesicles
Golgi Apparatus MESH: Microscopy Fluorescence Polymerase Chain Reaction Salivary Glands 0302 clinical medicine MESH: Animals MESH: Clathrin 0303 health sciences Granule (cell biology) Signal transducing adaptor protein Articles Secretory Vesicle Cell biology MESH: Adaptor Protein Complex 1 Protein Transport Drosophila melanogaster MESH: trans-Golgi Network MESH: Epithelial Cells symbols Clathrin adaptor proteins trans-Golgi Network MESH: Salivary Glands MESH: Protein Transport Adaptor Protein Complex 1 MESH: Microscopy Electron [SDV.BC]Life Sciences [q-bio]/Cellular Biology Biology Clathrin MESH: Drosophila melanogaster 03 medical and health sciences symbols.namesake MESH: Golgi Apparatus Animals [SDV.BC] Life Sciences [q-bio]/Cellular Biology Molecular Biology 030304 developmental biology Secretory Vesicles Epithelial Cells MESH: Polymerase Chain Reaction Cell Biology Golgi apparatus Microscopy Electron Secretory protein Microscopy Fluorescence Membrane Trafficking biology.protein 030217 neurology & neurosurgery Biogenesis |
| Zdroj: | Molecular Biology of the Cell Molecular Biology of the Cell, 2011, 22 (12), pp.2094-105. ⟨10.1091/mbc.E11-01-0054⟩ Molecular Biology of the Cell, American Society for Cell Biology, 2011, 22 (12), pp.2094-105. ⟨10.1091/mbc.E11-01-0054⟩ |
| ISSN: | 1939-4586 |
| DOI: | 10.1091/mbc.E11-01-0054⟩ |
| Popis: | Clathrin and AP-1 are required for the formation of mucin-type secretory granules in Drosophila larval salivary gland cells. Clathrin and AP-1 colocalize with secretory cargo at the trans-Golgi network (TGN) and on immature granules. Moreover, clathrin recruitment to the TGN requires AP-1. Strikingly, loss of AP-1 or clathrin profoundly blocks granule biogenesis. Regulated secretion of hormones, digestive enzymes, and other biologically active molecules requires the formation of secretory granules. Clathrin and the clathrin adaptor protein complex 1 (AP-1) are necessary for maturation of exocrine, endocrine, and neuroendocrine secretory granules. However, the initial steps of secretory granule biogenesis are only minimally understood. Powerful genetic approaches available in the fruit fly Drosophila melanogaster were used to investigate the molecular pathway for biogenesis of the mucin-containing “glue granules” that form within epithelial cells of the third-instar larval salivary gland. Clathrin and AP-1 colocalize at the trans-Golgi network (TGN) and clathrin recruitment requires AP-1. Furthermore, clathrin and AP-1 colocalize with secretory cargo at the TGN and on immature granules. Finally, loss of clathrin or AP-1 leads to a profound block in secretory granule formation. These findings establish a novel role for AP-1– and clathrin-dependent trafficking in the biogenesis of mucin-containing secretory granules. |
| Databáze: | OpenAIRE |
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