A rapid spin through oil results in higher cell-associated concentrations of antiretrovirals compared with conventional cell washing
| Autor: | Lee C. Winchester, Theodore J. Cory, Brian L. Robbins, Courtney V. Fletcher |
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| Rok vydání: | 2015 |
| Předmět: |
Drug
Efavirenz media_common.quotation_subject Clinical Biochemistry Pharmacology Mass Spectrometry Analytical Chemistry Cell Line chemistry.chemical_compound medicine Humans Centrifugation General Pharmacology Toxicology and Pharmaceutics Darunavir Chromatography High Pressure Liquid media_common Chromatography Chemistry Methodology virus diseases Lopinavir General Medicine HIV Protease Inhibitors Raltegravir Atazanavir Medical Laboratory Technology Anti-Retroviral Agents Isotope Labeling Ritonavir Isotonic Solutions Oils medicine.drug |
| Zdroj: | Bioanalysis. 7(12) |
| ISSN: | 1757-6199 |
| Popis: | Background: Determination of cell-associated antiretroviral drug concentrations is necessary for research into reservoirs of HIV. Variation exists in cell-associated drug concentrations among research groups. One cause for this may be washing cells during processing. We explored spinning cells through oil to minimize this variability. Methods & results: Raltegravir, atazanavir, darunavir, efavirenz, lopinavir and ritonavir concentrations were assessed in CEM.ss T cells washed with HBSS and oil-spun cells. Oil-spun cells had significantly higher concentrations for all drugs compared with samples washed with HBSS. Conclusion: The decline in cell-associated drug concentrations with saline washes compared with a single spin through oil shows the utility of a spin through oil. Oil centrifugation results in high cell-associated drug concentrations, and can be done in a fast, efficient manner. |
| Databáze: | OpenAIRE |
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