Cytogenetic damage and tumor incidence in mouse skin after single, topical applications of 7,12-dimethylbenz[a]anthracene
| Autor: | Antonio M. Bonin, Hartmut H. Steinel, Shuilin He, Robert S.U. Baker |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Keratinocytes
Skin Neoplasms 9 10-Dimethyl-1 2-benzanthracene Administration Topical Health Toxicology and Mutagenesis DMBA Biology medicine.disease_cause Mice chemistry.chemical_compound Genetics medicine Carcinoma Animals Molecular Biology Cells Cultured Micronuclei Chromosome-Defective Carcinogen Chromosome Aberrations Micronucleus Tests 7 12-Dimethylbenz[a]anthracene Aneuploidy medicine.disease chemistry Micronucleus test Cancer research Papilloma Micronucleus Genotoxicity |
| Zdroj: | Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 285:19-26 |
| ISSN: | 0027-5107 |
| DOI: | 10.1016/0027-5107(93)90047-j |
| Popis: | Micronucleus induction, chromosomal damage and aneuploidy were evaluated in whole skin keratinocyte cultures derived from HRA/Skh mice after single in vivo applications of 0.256, 2.56 and 25.6 μg (1, 10 and 100 nmoles) of the carcinogen, 7,12-dimethylbenz[ a ]anthracene (DMBA). These genotoxicity end-points were compared with papilloma and carcinoma occurrence at the same dose levels of carcinogen. While the lower 2 doses of DMBA significantly increased the incidence of micronuclei and other chromosomal anomalies in keratinocytes, the two highest doses resulted in a significantly increased papilloma yield (0.297 and 3.895 papillomas/mouse) and incidence (24.3 and 100%). Carcinomas appeared only at the highest dose (0.125 carcinomas/mouse; 5% incidence). Neither papillomas nor carcinomas occurred in solvent-treated control mice. None of the three applied doses induced aneuploidy under conditions leading to an increase in tumors and/or chromosomal damage. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|