Determination of the frequency, species distribution and antimicrobial resistance of staphylococci isolated from dogs and their owners in Trinidad

Autor: Khalil Ashraph, Karla Georges, Anisah Yusuf, Sharianne Suepaul, Jamie Sookhoo, Patrick Butaye, Chandrashekhar Unakal, Filip Boyen
Rok vydání: 2021
Předmět:
0301 basic medicine
Staphylococcus
HEALTHY DOGS
SUSCEPTIBILITY
Pathology and Laboratory Medicine
medicine.disease_cause
COLONIZATION
Antibiotics
Drug Resistance
Multiple
Bacterial
Medicine and Health Sciences
MOLECULAR CHARACTERIZATION
METHICILLIN RESISTANCE
Mammals
Multidisciplinary
Antimicrobials
COAGULASE-NEGATIVE STAPHYLOCOCCI
Sulfamethoxazole
Eukaryota
Drugs
Antimicrobial
Bacterial Pathogens
PREVALENCE
Trinidad and Tobago
Medical Microbiology
Tetracyclines
Staphylococcus aureus
Vertebrates
Medicine
Methicillin-resistant Staphylococcus aureus
Pathogens
Research Article
medicine.drug
Coagulase
Science
030106 microbiology
Biology
Microbiology
03 medical and health sciences
Dogs
Antibiotic resistance
Species Specificity
Microbial Control
Drug Resistance
Bacterial
medicine
Animals
Humans
PSEUDINTERMEDIUS MRSP
Veterinary Sciences
Staphylococcus Epidermidis
Microbial Pathogens
Pharmacology
Chi-Square Distribution
Bacteria
IDENTIFICATION
Staphylococcus intermedius
Ownership
Organisms
Biology and Life Sciences
Penicillin
biology.organism_classification
Trimethoprim
Multiple drug resistance
Chloramphenicol
030104 developmental biology
FLIGHT MASS-SPECTROMETRY
Amniotes
Antimicrobial Resistance
Zoology
Zdroj: PLOS ONE
PLoS ONE, Vol 16, Iss 7, p e0254048 (2021)
PLoS ONE
ISSN: 1932-6203
Popis: The close contact between humans and their dogs can lead to the commingling of staphylococci and the exchange of mobile genetic elements encoding antimicrobial resistance. The objectives of this study were to determine the species distribution and antimicrobial resistance patterns of staphylococci colonizing canine pets and their owners in Trinidad. Staphylococci were isolated from canine pets and their owners and identified using MALDI-TOF mass spectrometry. Antimicrobial susceptibilities were determined using the Kirby-Bauer disc diffusion method against seven classes of antimicrobial agents. A total of 440 staphylococci were isolated from 112 canine pets and their owners, 53.4% were from canine pets and 46.6% were from owners. Twenty-four species were detected, of which, most isolates (32.5%) belonged to the Staphylococcus intermedius group (SIG). S. sciuri was the most common species of coagulase-negative staphylococci (CoNS) comprising 22.3% of all isolates. Antimicrobial resistance was highest against commonly used antimicrobials, such as penicillin (51.4%), tetracycline (26.1%) and trimethoprim/sulfamethoxazole (18.6%). These antimicrobials also comprised the most common multidrug resistance (MDR) combination. Overall, 19.1% of isolates displayed multidrug resistance. No methicillin-resistant Staphylococcus aureus (MRSA) isolates were detected. However, methicillin resistance was detected in 13.3% and 15.1% of coagulase-positive staphylococci (CoPS) and the CoNS+CoVS (combined CoNS and coagulase-variable staphylococci) group respectively. The presence of methicillin-resistant staphylococci is worrisome because there is the potential for the transfer of these strains between dogs and humans. These strains may act as a reservoir of resistance genes.
Databáze: OpenAIRE
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