Dendritic cells inversely regulate airway inflammation in cigarette smoke-exposed mice
| Autor: | Ezzati Givi, Masoumeh, Akbari, Peyman, Boon, Louis, Puzovic, Vladimir S, Bezemer, Gillina F G, Ricciardolo, Fabio L M, Folkerts, Gert, Redegeld, Frank A, Mortaz, Esmaeil, Pharmacology, Sub Immunopharmacology, Sub Airway in vivo Pharmacology, Sub General Pharmacology |
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| Přispěvatelé: | Pharmacology, Sub Immunopharmacology, Sub Airway in vivo Pharmacology, Sub General Pharmacology |
| Rok vydání: | 2014 |
| Předmět: |
0301 basic medicine
Pulmonary and Respiratory Medicine Physiology Inflammation Pathogenesis 03 medical and health sciences 0302 clinical medicine Immune system Physiology (medical) medicine Animals Respiratory system Bronchitis Mice Inbred BALB C Lung medicine.diagnostic_test business.industry Interleukin-17 Smoking hemic and immune systems Cell Biology Dendritic Cells Pneumonia respiratory system Disease Models Animal 030104 developmental biology medicine.anatomical_structure Bronchoalveolar lavage Immunology Alveolar macrophage Female Interleukin 17 medicine.symptom business Bronchoalveolar Lavage Fluid 030215 immunology |
| Zdroj: | American journal of physiology. Lung cellular and molecular physiology, 310(1), L95. American Physiological Society |
| ISSN: | 1522-1504 1040-0605 |
| Popis: | The recruitment and activation of inflammatory cells into the respiratory system is considered a crucial feature in the pathophysiology of chronic obstructive pulmonary disease (COPD). Because dendritic cells (DCs) have a pivotal role in the onset and regulation of immune responses, we investigated the effect of modulating DC subsets on airway inflammation by acute cigarette smoke (CS) exposure. CS-exposed mice (5 days) were treated with fms-like tyrosine kinase 3 ligand (Flt3L) and 120g8 antibody to increase total DC numbers and deplete plasmacytoid DCs (pDCs), respectively. Flt3L treatment decreased the number of inflammatory cells in the bronchoalveolar lavage (BALF) of the smoke-exposed mice and increased these in lung tissue. DC modulation reduced IL-17 and increased IL-10 levels, which may be responsible for the suppression of the BALF cells. Furthermore, depletion of pDCs led to increased infiltration of alveolar macrophages while restricting the presence of CD103+ DCs. This study suggests that DC subsets may differentially and compartment-dependent influence the inflammation induced by CS. pDC may play a role in preventing the pathogenesis of CS by inhibiting the alveolar macrophage migration to lung and increasing CD103+ DCs at inflammatory sites to avoid extensive lung tissue damage. |
| Databáze: | OpenAIRE |
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