BAX -248 G>A and BCL2 -938 C>A Variant Lowers the Survival in Patients with Nasopharyngeal Carcinoma and Could be Associated with Tissue-Specific Malignancies: A Multi-Method Approach
| Autor: | Sandeep Ghatak, Eric Zomawia, Nilanjana Das, Komri Riba, R. Rajendra Reddy, Sudipa Mal, Arindom Chakraborty, Syamantak Mukherjee, Sushil Kumar Sahu, Zoreng puii, Amol Suryawanshi, Nabanita Roy Chattopadhyay, Shanmugam Rajasubramaniam, Tathagata Choudhuri, Yengkhom Indibor Singh, Ashok Kumar Das, Saikat De, Sankar Deb Roy, Koustav Chatterjee, Sam Tsering, Piyanki Das |
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| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty India computational study Context (language use) Single-nucleotide polymorphism Kaplan-Meier Estimate Gastroenterology 03 medical and health sciences symbols.namesake 0302 clinical medicine Internal medicine medicine Transcriptional regulation Humans Tissue specific Genetic Predisposition to Disease In patient BAX- BCL2 bcl-2-Associated X Protein polymorphism- SNP Sanger sequencing Nasopharyngeal Carcinoma Polymorphism Genetic business.industry Nasopharyngeal Neoplasms General Medicine medicine.disease Survival Rate meta-analysis 030104 developmental biology Proto-Oncogene Proteins c-bcl-2 Nasopharyngeal carcinoma Case-Control Studies 030220 oncology & carcinogenesis Meta-analysis symbols Female NPC business Research Article |
| Zdroj: | Asian Pacific Journal of Cancer Prevention : APJCP |
| ISSN: | 2476-762X |
| DOI: | 10.31557/apjcp.2021.22.4.1171 |
| Popis: | Background: The association of BAX -248 G>A and BCL2 -938 C>A with different cancers created conflicts. We studied the correlation and the effect of these polymorphisms in patients with Nasopharyngeal Carcinoma (NPC). Methods: PCR-RFLP and Sanger sequencing were used to detect polymorphisms. Statistical analysis including forest plot and Kaplan-Meier Log-rank test was conducted to investigate the association and effect of these SNPs on the NPC patients’ survival. The computational study was performed to investigate the possible regulatory role between these polymorphisms and the poor survival of NPC patients. Meta-analysis was executed to check the tissue-specific association of these polymorphisms in the context of global cancer prognosis. Results: We observed an increased and significant association of BAX -248 G>A [GA:OR=5.29, 95%CI=1.67,16.67, P=0.004; GA+AA:OR=5.71, 95%CI=1.82,17.90, P =0.002; A:OR=5.33, 95%CI=1.76,16.13, P=0.003], and BCL2 -938 C>A [CA:OR=2.26, 95%CI=1.03,4.96, P=0.04; AA:OR=3.56, 95%CI=0.97,13.05, P=0.05; CA+AA:OR=3.10, 95%CI=1.51,6.35, P=0.002; A:OR=2.90, 95% CI=1.59,5.29, P=0.0005] with the risk of NPC. Also, these SNPs were strongly correlated with poor survival in NPC patients (lower estimated survival mean, lower estimated proportion surviving at 5 years with pA showed a significant correlation with carcinomas [A vs G:OR=1.60, 95%CI=1.09,2.34, P=0.01; AA vs GG:OR=2.61, 95%CI=1.68,4.06, PA with other malignancies [A vs C:OR=1.45, 95%CI=1.26,1.66, PA and BCL2 -938 C>A was associated with poor survival in NPC patients. It may increase cancer susceptibility through transcriptional regulation. Moreover, these SNPs’ effects could be tissue-specific. |
| Databáze: | OpenAIRE |
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