The Legionella micdadei flagellin: Expression in Escherichia coli K 12 and DNA sequence of the gene
| Autor: | Geoffrey H. Shand, Niels Høiby, J. M. Bangsborg, Peter Hindersson |
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| Rok vydání: | 1995 |
| Předmět: |
DNA
Bacterial Microbiology (medical) Legionella micdadei Molecular Sequence Data Legionella medicine.disease_cause Pathology and Forensic Medicine Microbiology Plasmid Escherichia coli medicine Primer walking Immunology and Allergy Amino Acid Sequence Cloning Molecular Gene Base Sequence biology Nucleic acid sequence General Medicine biology.organism_classification Molecular biology biology.protein Tatlockia micdadei Flagellin |
| Zdroj: | APMIS. 103:869-877 |
| ISSN: | 1600-0463 0903-4641 |
| Popis: | To study the structure and function of the Legionella flagellum, we screened a genomic L. micdadei library in Escherichia coli for expression of the flagellin (Fla) subunit. One recombinant clone, JM105 (pHI5588), producing a truncated Fla protein of 40.5 kDa was identified. The plasmid pHI5588 carried a L. micdadei DNA insert of 5 kb, containing ca 95% of the fla gene. The complete DNA sequence of the L. micdadei fla gene was obtained by combining sequence data from pHI5588 with results using a polymerase chain reaction-based system for genome walking (vectorette PCR). The L. micdadei fla gene shared a high degree of homology with other flagellin genes in the amino- and carboxy termini, whereas the central region was found to be nonconserved. The fla sequence will facilitate the cloning of Fla proteins from other Legionella species and the study of flagella in the pathogenesis of Legionnaires' disease. |
| Databáze: | OpenAIRE |
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