Inhibition of the Dopamine D1 Receptor Signaling by PSD-95
| Autor: | Ole Isacson, Marc G. Caron, Jingping Zhang, Wei-Dong Yao, Angel Viñuela, Seth G. N. Grant, Penelope J. Hallett, Gregory M. Miller, Mark H. Neely |
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| Rok vydání: | 2007 |
| Předmět: |
Dynamins
Agonist Dendritic spine medicine.drug_class Dendritic Spines Dopamine Agents Gene Expression Biology Biochemistry Article Mice Dopamine receptor D1 Cyclic AMP medicine Animals Humans Learning Receptor Molecular Biology Mice Knockout Behavior Animal Mental Disorders Receptors Dopamine D1 Dopaminergic Intracellular Signaling Peptides and Proteins Membrane Proteins Cell Biology Endocytosis Protein Structure Tertiary Cell biology Amphetamine Protein Transport Receptors Glutamate Dopamine receptor Signal transduction Disks Large Homolog 4 Protein Guanylate Kinases Postsynaptic density Locomotion Protein Binding Signal Transduction |
| Zdroj: | Journal of Biological Chemistry. 282:15778-15789 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m611485200 |
| Popis: | Dopamine D1 receptors play an important role in movement, reward, and learning and are implicated in a number of neurological and psychiatric disorders. These receptors are concentrated in dendritic spines of neurons, including the spine head and the postsynaptic density. D1 within spines is thought to modulate the local channels and receptors to control the excitability and synaptic properties of spines. The molecular mechanisms mediating D1 trafficking, anchorage, and function in spines remain elusive. Here we show that the synaptic scaffolding protein PSD-95 thought to play a role in stabilizing glutamate receptors in the postsynaptic density, interacts with D1 and regulates its trafficking and function. Interestingly, the D1-PSD-95 interaction does not require the well characterized domains of PSD-95 but is mediated by the carboxyl-terminal tail of D1 and the NH(2) terminus of PSD-95, a region that is recognized only recently to participate in protein-protein interaction. Co-expression of PSD-95 with D1 in mammalian cells inhibits the D1-mediated cAMP accumulation without altering the total expression level or the agonist binding properties of the receptor. The diminished D1 signaling is mediated by reduced D1 expression at the cell surface as a consequence of an enhanced constitutive, dynamin-dependent endocytosis. In addition, genetically engineered mice lacking PSD-95 show a heightened behavioral response to either a D1 agonist or the psychostimulant amphetamine. These studies demonstrate a role for a glutamatergic scaffold in dopamine receptor signaling and trafficking and identify a new potential target for the modulation of abnormal dopaminergic function. |
| Databáze: | OpenAIRE |
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