Pre-existing astrocytes form functional perisynaptic processes on neurons generated in the adult hippocampus

Autor: Valentin Schmutz, Nicolas Toni, Mari A. Virtanen, Lucas A. Mongiat, Jan Armida, Matteo Bergami, Laszlo Vutskits, Marine Krzisch, Fred H. Gage, Alejandro F. Schinder, Jacqueline Kocher-Braissant, Silvio Gabriel Temprana, Karl-Klaus Conzelmann, Rudolf Kraftsik
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Dendritic spine
Patch-Clamp Techniques
Synaptogenesis
Hippocampus
Hippocampal formation
Adult neurogenesis
Synaptic Transmission
Mice
Microscopy
Immunoelectron
Neurons
Kainic Acid
Microscopy
Confocal
ddc:617
General Neuroscience
Neurogenesis
purl.org/becyt/ford/3.1 [https]
Cell biology
Isoenzymes
Medicina Básica
Excitatory postsynaptic potential
purl.org/becyt/ford/3 [https]
Original Article
Anatomy
Histology
CIENCIAS MÉDICAS Y DE LA SALUD
Neuroscience(all)
Dendritic Spines
Green Fluorescent Proteins
Neurociencias
Animals
Astrocytes/physiology
Astrocytes/ultrastructure
Bromodeoxyuridine/metabolism
Dendritic Spines/drug effects
Dendritic Spines/metabolism
Excitatory Postsynaptic Potentials/drug effects
Excitatory Postsynaptic Potentials/genetics
Gene Expression Regulation/genetics
Glial Fibrillary Acidic Protein/genetics
Glial Fibrillary Acidic Protein/metabolism
Green Fluorescent Proteins/genetics
Green Fluorescent Proteins/metabolism
Hippocampus/cytology
Isoenzymes/genetics
Isoenzymes/metabolism
Kainic Acid/analogs & derivatives
Kainic Acid/pharmacology
Mice
Inbred C57BL
Mice
Transgenic
Neurogenesis/drug effects
Neurogenesis/genetics
Neurons/cytology
Neurons/drug effects
Phosphopyruvate Hydratase/metabolism
Retinal Dehydrogenase/genetics
Retinal Dehydrogenase/metabolism
S100 Calcium Binding Protein beta Subunit/metabolism
Synapses/physiology
Synapses/ultrastructure
Synaptic Transmission/drug effects
Synaptic Transmission/genetics
S100 Calcium Binding Protein beta Subunit
Biology
Neurotransmission
Aldehyde Dehydrogenase 1 Family
Glial Fibrillary Acidic Protein
Dentate gyrus
Excitatory Postsynaptic Potentials
Retinal Dehydrogenase
ddc:616.8
Bromodeoxyuridine
Gene Expression Regulation
Phosphopyruvate Hydratase
Astrocytes
Synapses
Perisynaptic processes
Neuroscience
Zdroj: Brain Structure & Function
Brain structure & function
Brain Structure and Function, vol. 220, no. 4, pp. 2027-2042
CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
Brain Structure and Function, Vol. 220, No 4 (2015) pp. 2027-2042
ISSN: 1863-2653
Popis: The adult dentate gyrus produces new neurons that morphologically and functionally integrate into the hippocampal network. In the adult brain, most excitatory synapses are ensheathed by astrocytic perisynaptic processes that regulate synaptic structure and function. However, these processes are formed during embryonic or early postnatal development and it is unknown whether astrocytes can also ensheathe synapses of neurons born during adulthood and, if so, whether they play a role in their synaptic transmission. Here, we used a combination of serial-section immuno-electron microscopy, confocal microscopy, and electrophysiology to examine the formation of perisynaptic processes on adult-born neurons. We found that the afferent and efferent synapses of newborn neurons are ensheathed by astrocytic processes, irrespective of the age of the neurons or the size of their synapses. The quantification of gliogenesis and the distribution of astrocytic processes on synapses formed by adult-born neurons suggest that the majority of these processes are recruited from pre-existing astrocytes. Furthermore, the inhibition of astrocytic glutamate re-uptake significantly reduced postsynaptic currents and increased paired-pulse facilitation in adult-born neurons, suggesting that perisynaptic processes modulate synaptic transmission on these cells. Finally, some processes were found intercalated between newly formed dendritic spines and potential presynaptic partners, suggesting that they may also play a structural role in the connectivity of new spines. Together, these results indicate that pre-existing astrocytes remodel their processes to ensheathe synapses of adult-born neurons and participate to the functional and structural integration of these cells into the hippocampal network. Fil: Krzisch, Marine. University of Lausanne. Department of Fundamental Neurosciences; Suiza Fil: Temprana, Silvio Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina Fil: Mongiat, Lucas Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina Fil: Armida, Jan. University of Lausanne. Department of Fundamental Neurosciences; Suiza Fil: Schmutz, Valentin. University of Lausanne. Department of Fundamental Neurosciences; Suiza Fil: Virtanen, Mari A.. Universidad de Ginebra; Suiza Fil: Kocher Braissant, Jacqueline. University of Lausanne. Department of Fundamental Neurosciences; Suiza Fil: Kraftsik, Rudolf. University of Lausanne. Department of Fundamental Neurosciences; Suiza Fil: Vutskits, Laszlo. Universidad de Ginebra; Suiza. University Hospital of Geneva. Department of Anesthesiology, Pharmacology and Intensive Care; Suiza Fil: Conzelmann, Karl Klaus. Ludwig-Maximilians University Múnich. Max von Pettenkofer Institute and Gene Center; Alemania Fil: Bergami, Matteo. University Hospital of Cologne; Alemania Fil: Gage, Fred H.. Salk Institute for Biological Studies; Estados Unidos Fil: Schinder, Alejandro Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina Fil: Toni, Nicolas. University of Lausanne. Department of Fundamental Neurosciences; Suiza
Databáze: OpenAIRE
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