Time-lapse imaging reveals highly dynamic structural maturation of postnatally born dentate granule cells in organotypic entorhino-hippocampal slice cultures
| Autor: | Andreas Vlachos, Marcel Beining, Thomas Deller, Hermann Cuntz, Tijana Radic, Stephan W. Schwarzacher, Tassilo Jungenitz, Mathias Singer |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Time Factors Neurogenesis Hippocampal slice Genetic Vectors Gene Expression Post injection Hippocampal formation Biology Hippocampus Time-Lapse Imaging Article Mice 03 medical and health sciences 0302 clinical medicine Genes Reporter Transduction Genetic Animals ddc:610 Multidisciplinary Dentate gyrus Granule (cell biology) Immunohistochemistry In vitro Anterograde tracing 030104 developmental biology Animals Newborn Dentate Gyrus sense organs Neuroscience Biomarkers 030217 neurology & neurosurgery hormones hormone substitutes and hormone antagonists |
| Zdroj: | Sci Rep Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep43724 |
| Popis: | Neurogenesis of hippocampal granule cells (GCs) persists throughout mammalian life and is important for learning and memory. How newborn GCs differentiate and mature into an existing circuit during this time period is not yet fully understood. We established a method to visualize postnatally generated GCs in organotypic entorhino-hippocampal slice cultures (OTCs) using retroviral (RV) GFP-labeling and performed time-lapse imaging to study their morphological development in vitro. Using anterograde tracing we could, furthermore, demonstrate that the postnatally generated GCs in OTCs, similar to adult born GCs, grow into an existing entorhino-dentate circuitry. RV-labeled GCs were identified and individual cells were followed for up to four weeks post injection. Postnatally born GCs exhibited highly dynamic structural changes, including dendritic growth spurts but also retraction of dendrites and phases of dendritic stabilization. In contrast, older, presumably prenatally born GCs labeled with an adeno-associated virus (AAV), were far less dynamic. We propose that the high degree of structural flexibility seen in our preparations is necessary for the integration of newborn granule cells into an already existing neuronal circuit of the dentate gyrus in which they have to compete for entorhinal input with cells generated and integrated earlier. |
| Databáze: | OpenAIRE |
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