A microfluidic chip based model for the study of full thickness human intestinal tissue using dual flow
| Autor: | Amy Dawson, John Greenman, J. Macfie, C. Dyer, J. Davies, Morten Jacobsen, L. Karsai |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Biomedical Engineering 02 engineering and technology Biology Haematoxylin Inflammatory bowel disease 03 medical and health sciences chemistry.chemical_compound Colloid and Surface Chemistry medicine General Materials Science Fluid Flow and Transfer Processes Eosin Errata 021001 nanoscience & nanotechnology Condensed Matter Physics medicine.disease Staining 030104 developmental biology chemistry Cell culture Calprotectin 0210 nano-technology Perfusion Ex vivo |
| Zdroj: | Biomicrofluidics. 10(6) |
| ISSN: | 1932-1058 |
| Popis: | The study of inflammatory bowel disease, including Ulcerative Colitis and Crohn's Disease, has relied largely upon the use of animal or cell culture models; neither of which can represent all aspects of the human pathophysiology. Presented herein is a dual flow microfluidic device which holds full thickness human intestinal tissue in a known orientation. The luminal and serosal sides are independently perfused ex vivo with nutrients with simultaneous waste removal for up to 72 h. The microfluidic device maintains the viability and integrity of the tissue as demonstrated through Haematoxylin & Eosin staining, immunohistochemistry and release of lactate dehydrogenase. In addition, the inflammatory state remains in the tissue after perfusion on the device as determined by measuring calprotectin levels. It is anticipated that this human model will be extremely useful for studying the biology and testing novel interventions in diseased tissue. |
| Databáze: | OpenAIRE |
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