In vitro antioxidant activity, total phenolic and flavonoid contents of ethanol extract of stem and leaf of Grewia carpinifolia
| Autor: | Zeng Guang-Zhi, F. O. Olayemi, Olamide E. Adebiyi, Tan Ning-Hua |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Antioxidant DPPH medicine.medical_treatment Pharmaceutical Science Medicine (miscellaneous) Grewia carpinifolia 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Lipid oxidation Antioxidant activity TBARS medicine ABTS Food science Gallic acid lcsh:Science lcsh:R5-920 biology 010401 analytical chemistry Ascorbic acid biology.organism_classification Agricultural and Biological Sciences (miscellaneous) 0104 chemical sciences 030104 developmental biology Grewia Biochemistry chemistry FRAP lcsh:Q lcsh:Medicine (General) |
| Zdroj: | Beni-Suef University Journal of Basic and Applied Sciences, Vol 6, Iss 1, Pp 10-14 (2017) |
| ISSN: | 2314-8535 |
| Popis: | Free radicals are reactive molecules involved in many physiological processes and have been associated with many diseases, such as cancer, arthritis and liver injury. As a result, there is need to explore substances with free radical scavenging and or antioxidant activity. The present study was designed to evaluate the free radical scavenging activity of ethanol extract of leaf and stem of Grewia carpinifolia using various in vitro models. Ascorbic acid was used as the reference in the study. 1,1-Diphenyl-2-picryl hydroxyl (DPPH) quenching assay, 2,2′-azinobis-3-ethylbenzothiozoline-6-sulfonic acid (ABTS) cation decolorization test, ferric reducing antioxidant power (FRAP) Assay systems were selected for the present experiment. The ability of the extracts to inhibit lipid oxidation was measured using Thiobarbituric Acid Reactive Substances (TBARS) assay. The extracts were used at 0.2, 0.4, 0.6, 0.8 and 1 mg/ml concentrations and radical scavenging activity was determined in terms of inhibition percentage. The IC50 (concentration required for 50% inhibition) was also calculated for each radical. The study revealed that Grewia carpinifolia has a high radical scavenging activity in the various radical systems. The total phenolic content was 19.08 ± 1.21 mg gallic acid equivalent (GAE)/g extract and 14.85 ± 1.09 mg GAE/g extract for the leaf and stem respectively while the flavonoid content was 9.00 ± 0.13 and 13.22 ± 1.53 mg quercetin/g extract. The antioxidant activity of Grewia carpinifolia extract may be due to the high level of flavonoids and phenols in the plant. |
| Databáze: | OpenAIRE |
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