The prevalence of Chlamydia trachomatis in fresh tissue specimens from patients with ectopic pregnancy or tubal factor infertility as determined by PCR and in-situ hybridisation
| Autor: | Gordon Narayansingh, Samuel S. Ramsewak, Rachael E. L. Barlow, Ian D. Cooke, Mark K. Heatley, Julian M. Jenkins, O.A. Odukoya, Adrian Eley |
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| Rok vydání: | 2001 |
| Předmět: |
Microbiology (medical)
Adult DNA Bacterial medicine.medical_specialty Chlamydia trachomatis Endometrium medicine.disease_cause Microbiology Polymerase Chain Reaction law.invention law Pregnancy medicine Prevalence Humans Chlamydiaceae Polymerase chain reaction In Situ Hybridization Gynecology biology Ectopic pregnancy General Medicine Genitalia Female Tubal factor infertility Chlamydia Infections Fallopian Tube Diseases Middle Aged biology.organism_classification medicine.disease United Kingdom Pregnancy Ectopic medicine.anatomical_structure Trinidad and Tobago Chlamydiales Female Infertility Female Fallopian tube |
| Zdroj: | Journal of medical microbiology. 50(10) |
| ISSN: | 0022-2615 |
| Popis: | The prevalence of chlamydial DNA determined by PCR and in-situ hybridisation (ISH) in fresh tissue specimens (endometrium, fallopian tube and ovary) was investigated in 33 women presenting with ectopic pregnancy (EP), 14 women with tubal factor infertility (TFI) and 50 control patients from the UK and the West Indies. In the UK EP group, chlamydial DNA was detected by PCR in 56% of patients; similar results were found in the Trinidad EP group (67%). In the TFI group, chlamydial DNA was detected in (71%) of patients by PCR. The detection of Chlamydia trachomatis DNA by ISH was highest in the TFI group (43%). Women presenting with EP and TFI showed evidence of previous or current genital C. trachomatis infection, underlining the importance of this micro-organism in the development of these conditions. Importantly, chlamydial DNA could be detected in DNA preparations from the endometrium, fallopian tube and ovary of EP and TFI patients at the time of surgery. |
| Databáze: | OpenAIRE |
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