Photodynamic activity of Temoporfin nanoparticles induces a shift to the M1-like phenotype in M2-polarized macrophages
| Autor: | Rudolf W. Steiner, Ralf-Uwe Peter, Arno Wiehe, Zhenxin Zhu, Rainer Wittig, Anastasia V. Ryabova, Claudia Scalfi-Happ, Victor B. Loschenov, Susanna Gräfe |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Light medicine.medical_treatment Biophysics Macrophage polarization Photodynamic therapy Tumor-associated macrophage Cell Line Temoporfin 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine In vivo medicine Humans Radiology Nuclear Medicine and imaging Photosensitizer Tissue homeostasis Radiation Photosensitizing Agents Radiological and Ultrasound Technology Monocyte Macrophages Cell Polarity Cell Differentiation Cell biology 030104 developmental biology medicine.anatomical_structure Phenotype chemistry Mesoporphyrins 030220 oncology & carcinogenesis Nanoparticles |
| Zdroj: | Journal of photochemistry and photobiology. B, Biology. 185 |
| ISSN: | 1873-2682 |
| Popis: | The monocyte/macrophage cell lineage reveals an enormous plasticity, which is required for tissue homeostasis, but is also undermined in various disease states, leading to a functional involvement of macrophages in major human diseases such as atherosclerosis and cancer. We recently generated in vivo evidence that crystalline, nonfluorescent nanoparticles of the hydrophobic porphyrin-related photosensitizer Aluminum phthalocyanine are selectively dissolved and thus may be used for specific fluorescent labelling of rejected, but not of accepted xenotransplants. This led us to hypothesize that nanoparticles made of planar photosensitizers such as porphyrins and chlorins were preferentially taken up and dissolved by macrophages, which was verified by in vitro studies. Here, using an in vitro system for macrophage differentiation/polarization of the human monocyte THP-1 cell line, we demonstrate differential uptake/dissolution of Temoporfin-derived nanoparticles in polarized macrophages, which resulted in differential photosensitivity. More importantly, low dose photodynamic sensitization using Temoporfin nanoparticles can be used to trigger M1 re-polarization of THP-1 cells previously polarized to the M2 state. Thus, sublethal photodynamic treatment using Temoporfin nanoparticles might be applied to induce a phenotypic shift of tumor-associated macrophages for the correction of an immunosuppressive microenvironment in the treatment of cancer, which may synergize with immune checkpoint inhibition. |
| Databáze: | OpenAIRE |
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