Standardization and application of microsatellite markers for variety identification in tomato and wheat
| Autor: | Ronald Peeters, R. J. Cooke, Ben Vosman, G.M.M. Bredemeijer, Martin W. Ganal, P. Isaac |
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| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
STMS markers
Standardization business.industry food and beverages Microsatellite Network Horticulture Biology Crop species Tomato Biotechnology PRI Biodiversity and Breeding Database chemistry.chemical_compound chemistry PRI Biodiversiteit en Veredeling Molecular marker Wheat Standard test Identification (biology) business Analysis method |
| Zdroj: | Acta Horticulturae 546 (2001) Acta Horticulturae, 546, 307-316 |
| ISSN: | 0567-7572 |
| Popis: | The present study is part of a EU project that aims to demonstrate the technical viability of STMS markers for variety identification. As examples two important European crop species, tomato and wheat were chosen. Initially, about 30-40 STMS markers were used to identify a set of 20 good markers per crop and to standardise the methodology and the interpretation of the results in different laboratories. Several systems were used for the detection of STMS polymorphisms. The selected STMS markers are being tested on 500 varieties of each species and databases are being constructed. The first comparisons of data generated by the different laboratories revealed a high degree of agreement. The causes of discrepancies between duplicate samples analysed in different laboratories and precautions to prevent them, are discussed. |
| Databáze: | OpenAIRE |
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