Mimicking cellular transport mechanism in stem cells through endosomal escape of new peptide-coated quantum dots
| Autor: | Subramanian Tamil Selvan, Jackie Y. Ying, Sohail Ahmed, Karthikeyan Narayanan, Thankiah Sudhaharan, Swee Kuan Yen, Qingqing Dou, Parasuraman Padmanabhan |
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| Přispěvatelé: | Lee Kong Chian School of Medicine (LKCMedicine) |
| Rok vydání: | 2013 |
| Předmět: |
Endosome
Molecular Sequence Data Nuclear Localization Signals Plasma protein binding Endosomes Biology Microtubules Article Microtubule Quantum Dots Humans Science::Medicine [DRNTU] Amino Acid Sequence Peptide sequence Cell Nucleus Multidisciplinary Microscopy Confocal Biological Transport Mesenchymal Stem Cells Cell biology Transport protein Solubility Drug delivery Stem cell Peptides Nuclear localization sequence Protein Binding |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Protein transport is an important phenomenon in biological systems. Proteins are transported via several mechanisms to reach their destined compartment of cell for its complete function. One such mechanism is the microtubule mediated protein transport. Up to now, there are no reports on synthetic systems mimicking the biological protein transport mechanism. Here we report a highly efficient method of mimicking the microtubule mediated protein transport using newly designed biotinylated peptides encompassing a microtubule-associated sequence (MTAS) and a nuclear localization signaling (NLS) sequence, and their final conjugation with streptavidin-coated CdSe/ZnS quantum dots (QDs). Our results demonstrate that these novel bio-conjugated QDs enhance the endosomal escape and promote targeted delivery into the nucleus of human mesenchymal stem cells via microtubules. Mimicking the cellular transport mechanism in stem cells is highly desirable for diagnostics, targeting and therapeutic applications, opening up new avenues in the area of drug delivery. ASTAR (Agency for Sci., Tech. and Research, S’pore) Published version |
| Databáze: | OpenAIRE |
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