Activation of junB and c-myc primary response genes by interleukin 9 in a human factor-dependent cell line
| Autor: | Li Ya Kang, Yu Chung Yang |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Physiology
JUNB Lactams Macrocyclic Clinical Biochemistry Genes myc Biology Genes jun hemic and lymphatic diseases Gene expression Benzoquinones Tumor Cells Cultured Humans Interleukin 9 Protein kinase A Growth Substances Protein Kinase Inhibitors Protein kinase C Protein Kinase C Messenger RNA Cell growth Interleukin-9 Quinones Cell Biology Molecular biology Genistein Isoflavones Recombinant Proteins Gene Expression Regulation Rifabutin Tetradecanoylphorbol Acetate Signal transduction Signal Transduction |
| Zdroj: | Journal of cellular physiology. 163(3) |
| ISSN: | 0021-9541 |
| Popis: | Interleukin 9 (IL-9) stimulates the proliferation of various hematopoietic cell types. To elucidate the molecular mechanisms underlying the cell proliferation action, immediate-early gene expression elicited by IL-9 in a human factor-dependent cell line, MO7e, was studied. IL-9 stimulation resulted in a rapid and transient elevation of primary response genes including junB and c-myc. The differential effects of protein kinase inhibitors, herbimycin A, genistein, and H-7 on the steady-state mRNA level and the transcription rate of junB and c-myc genes triggered by IL-9 were also investigated. Herbimycin A, but not genistein, specifically inhibited the expression of junB steady-state mRNA and the in vitro transcription of the junB gene. IL-9-enhanced c-myc gene expression was completely inhibited by both herbimycin A and genistein at the level of transcriptional initiation. H-7 failed to inhibit c-myc, but partially abolished junB mRNA induction. The role of protein kinase C in IL-9-mediated junB induction was also examined. The different responses of junB and c-myc messages to protein kinase inhibitors suggested that more than one pathway may be involved in IL-9-mediated signal transduction which leads to the expression of junB and c-myc genes. |
| Databáze: | OpenAIRE |
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