Blood oxidative stress and post-exercise recovery are unaffected byhypobaric and hypoxic environments
| Autor: | Brent C. Ruby, Tiffany S. Quindry, Cassie M. Williamson-Reisdorph, Dustin Slivka, John C. Quindry, Kathryn G.S. Tiemessen, Walter S. Hailes, John S. Cuddy |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Adult
Male medicine.medical_specialty Adolescent 030209 endocrinology & metabolism Physical Therapy Sports Therapy and Rehabilitation medicine.disease_cause Antioxidants 03 medical and health sciences Recovery period Young Adult 0302 clinical medicine Heart Rate Internal medicine Post exercise Medicine Humans Orthopedics and Sports Medicine Hypoxia Muscle Skeletal Exercise chemistry.chemical_classification Reactive oxygen species business.industry Altitude Gene Expression Profiling 030229 sport sciences Hypoxia (medical) Oxygen Oxidative Stress Endocrinology Atmospheric Pressure chemistry Female medicine.symptom business Oxidative stress Biomarkers |
| Zdroj: | Journal of sports sciences. 39(12) |
| ISSN: | 1466-447X |
| Popis: | Hypobaria and hypoxia exert independent effects on oxidative stress during exercise, while combined effectson the post-exercise recovery period remain unclear.Accordingly, this study examined the recovery period during lab-simulated hypoxic and hypobaric conditions following exercise-induced oxidative stress. Participants (n=13) performed 60-minutes of cycling (70% watts max) in a normobaric normoxic environment followed by a four-hour recovery under three conditions; 1000m normobaric normoxia (NN, 675mmHg), 4400m normobaric hypoxia (NH, 675mmHg), or 4400m hypobaric hypoxia (HH, 440mmHg). Blood samples collected at Pre, Post, 2-Hours (2-HR), and 4-Hours (4-HR) post-exercise were analyzed fora potential increase in biochemical modifications of proteins(protein carbonyls, PC; 3-nitrotyrosines, 3NT) lipids (lipid hydroperoxides, LOOH; 8-isoprostanes, 8-ISO), and antioxidant capacity (FRAP, TEAC). Gene transcripts (EPAS, HMOX1, SOD2, NFE2L2) were quantified by qRT-PCR from muscle biopsies taken Pre and Post exercise. Hypoxia and hypobaria had no effect throughout recovery. Post-exercise TEAC (p=0.041), FRAP (p=0.013), and 8-ISO (p=0.044) increased, while PC (p=0.002) and 3-NT (p=0.032) were decreased. LOOH was lower in Post (p=0.018) NH trial samples. Exercise-dependent increases occurred in NFE2L2 (p=0.003), HMXO1 (p0.001), SOD2 (p=0.046), and EPAS (p=0.038). Exercise recovery under conditions of NH and HH did not impact blood oxidative stress or redox-sensitive gene transcripts. |
| Databáze: | OpenAIRE |
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