Reconstitution of cell migration at a glance
| Autor: | Juan Manuel Garcia-Arcos, Matthieu Piel, Aastha Mathur, Pablo J. Sáez, Renaud Chabrier, Lucie Barbier, Guilherme Pedreira de Freitas Nader, Mathieu Deygas, Pablo Vargas |
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| Přispěvatelé: | Stress génotoxiques et cancer, Université Paris-Sud - Paris 11 (UP11)-Institut Curie [Paris]-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche en Cancérologie de Lyon (UNICANCER/CRCL), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre d'Etudes et d'Expertise sur les Risques, l'Environnement, la Mobilité et l'Aménagement - Equipe-projet TEAM (Cerema Equipe-projet TEAM), Centre d'Etudes et d'Expertise sur les Risques, l'Environnement, la Mobilité et l'Aménagement (Cerema), Immunité et cancer, Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Curie [Paris], Compartimentation et dynamique cellulaires (CDC), Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]-Université Pierre et Marie Curie - Paris 6 (UPMC) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Cell
Microfluidics Cellular functions Biology Models Biological 03 medical and health sciences 0302 clinical medicine Cell Movement [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB] medicine Humans Dimethylpolysiloxanes ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences Neurogenesis Molecular Mimicry Cell migration Cell Biology Cell Microenvironment Cell biology Extracellular Matrix medicine.anatomical_structure Tissue remodeling Eukaryotic Cells Cellular Microenvironment Cancer cell Microtechnology Biological Assay Collagen Single-Cell Analysis 030217 neurology & neurosurgery Micropatterning |
| Zdroj: | Journal of Cell Science Journal of Cell Science, Company of Biologists, 2019, 132 (4), pp.jcs225565. ⟨10.1242/jcs.225565⟩ |
| ISSN: | 0021-9533 1477-9137 |
| DOI: | 10.1242/jcs.225565⟩ |
| Popis: | Single cells migrate in a myriad of physiological contexts, such as tissue patrolling by immune cells, and during neurogenesis and tissue remodeling, as well as in metastasis, the spread of cancer cells. To understand the basic principles of single-cell migration, a reductionist approach can be taken. This aims to control and deconstruct the complexity of different cellular microenvironments into simpler elementary constrains that can be recombined together. This approach is the cell microenvironment equivalent of in vitro reconstituted systems that combine elementary molecular players to understand cellular functions. In this Cell Science at a Glance article and accompanying poster, we present selected experimental setups that mimic different events that cells undergo during migration in vivo. These include polydimethylsiloxane (PDMS) devices to deform whole cells or organelles, micro patterning, nano-fabricated structures like grooves, and compartmentalized collagen chambers with chemical gradients. We also outline the main contribution of each technique to the understanding of different aspects of single-cell migration. |
| Databáze: | OpenAIRE |
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