A T‐cell diagnostic test for cystic echinococcosis based on Antigen B peptides

Autor: Antonella Teggi, M. A. Gomez Morales, Massimo Amicosante, Delia Goletti, Giuseppe Ippolito, Alessandra Ludovisi, Linda Petrone, Edoardo Pozio, Valentina Vanini, Angela Corpolongo
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Male
Pathology
medicine.medical_treatment
0302 clinical medicine
antigens
cytokine
antibodies
echinococcus spp
elisa/enzyme-linked immunosorbent assay
hydatidosis
immunodiagnosis
adult
aged
animals
antibodies
helminth
antigens
helminth
diagnostic tests
routine
echinococcosis
echinococcus granulosus
enzyme-linked immunosorbent assay
female
helminth proteins
humans
immunologic tests
interleukin-4
lipoproteins
male
middle aged
protein domains
sensitivity and specificity
Echinococcus granulosus
helminth
biology
Antigen b
Interleukin
Helminth Proteins
Middle Aged
3. Good health
Cytokine
medicine.anatomical_structure
routine
Original Article
Female
Adult
medicine.medical_specialty
Protein subunit
T cell
Lipoproteins
030231 tropical medicine
Immunology
Antibodies
Helminth
Enzyme-Linked Immunosorbent Assay
Immunologic Tests
Sensitivity and Specificity
03 medical and health sciences
diagnostic tests
Protein Domains
Echinococcosis
Echinococcus spp
medicine
Animals
Humans
Aged
Receiver operating characteristic
Cystic echinococcosis
Diagnostic Tests
Routine
ELISA/enzyme‐linked immunosorbent assay
Original Articles
biology.organism_classification
Molecular biology
030104 developmental biology
Antigens
Helminth
Parasitology
Interleukin-4
Zdroj: Parasite Immunology
ISSN: 1365-3024
0141-9838
Popis: Summary Cystic echinococcosis (CE) immunodiagnosis is still imperfect. We recently set‐up a whole‐blood test based on the interleukin (IL)‐4 response to the native Antigen B (AgB) of Echinococcus granulosus. However, AgB is encoded by a multigene family coding for five putative subunits. Therefore, the aims of this study were to analyse the IL‐4 response to peptides spanning the immunodominant regions of the five AgB subunits and to evaluate the accuracy of this assay for CE diagnosis. Peptides corresponding to each subunit were combined into five pools. A pool containing all peptides was also used (total pool). IL‐4 evaluated by enzyme‐linked immunosorbent assay was significantly higher in patients with CE compared to those without (NO‐CE subjects) when whole‐blood was stimulated with AgB1 and with the total pool. Moreover, IL‐4 levels in response to the total pool were significantly increased in patients with active cysts. Receiver Operator Curve analysis identified a cut‐off point of 0.59 pg/mL predicting active cysts diagnosis with 71% sensitivity and 82% specificity in serology‐positive CE patients. These data, if confirmed in a larger cohort, offer the opportunity to develop new diagnostic tools for CE based on a standardized source of AgB as the peptides.
Databáze: OpenAIRE
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