The rolC gene increases caffeoylquinic acid production in transformed artichoke cells
| Autor: | Viacheslav G. Rybin, Y. N. Zhuravlev, O. G. Koren, Yuliya V. Vereshchagina, N. H. T. Phan, G. K. Tchernoded, Victor P. Bulgakov, V. P. Grigorchuk, N. T. Minh, Tatiana Y. Gorpenchenko, L. T. Chau, G.N. Veremeichik |
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| Rok vydání: | 2014 |
| Předmět: |
Cynara
Quinic Acid General Medicine Biology Asteraceae biology.organism_classification Applied Microbiology and Biotechnology chemistry.chemical_compound Caffeoylquinic acid Chlorogenic acid chemistry Dry weight Cinnamates Cynara scolymus Botany Inducer Food science Secondary metabolism Gene Plant Proteins Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 98:7773-7780 |
| ISSN: | 1432-0614 0175-7598 |
| Popis: | Caffeoylquinic acids are found in artichokes, and they are currently considered important therapeutic or preventive agents for treating Alzheimer’s disease and diabetes. We transformed artichoke [the cultivated cardoon or Cynara cardunculus var. altilis DC (Asteraceae)] with the rolC gene, which is a known inducer of secondary metabolism. High-performance liquid chromatography with UV and high-resolution mass spectrometry (HPLC-UV-HRMS) revealed that the predominant metabolites synthesized in the transgenic calli were 1,5-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, and chlorogenic acid. The rolC-transformed calli contained 1.5 % caffeoylquinic acids by dry weight. The overall production of these metabolites was three times higher than that of the corresponding control calli. The enhancing effect of rolC remained stable over long-term cultivation. |
| Databáze: | OpenAIRE |
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