Effect of vitamin D3 on chemokine expression in pulmonary tuberculosis
| Autor: | Paramasivam Selvaraj, M. Harishankar, M. S. Jawahar, V.V. Banurekha, Brijendra Singh |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Adult
Vitamin Chemokine Vitamin D-binding protein Immunology Gene Expression CCL4 Chemokine CXCL9 Biochemistry CCL5 Young Adult chemistry.chemical_compound Bacterial Proteins Vitamin D and neurology Humans Immunology and Allergy CXCL10 Chemokine CCL4 Chemokine CCL5 Tuberculosis Pulmonary Molecular Biology Cells Cultured Chemokine CCL2 Chemokine CCL3 Cholecalciferol biology Reverse Transcriptase Polymerase Chain Reaction Macrophages Vitamins Hematology Molecular biology Chemokine CXCL10 chemistry Leukocytes Mononuclear biology.protein CXCL9 Chemokines |
| Zdroj: | Cytokine. 60:212-219 |
| ISSN: | 1043-4666 |
| DOI: | 10.1016/j.cyto.2012.06.238 |
| Popis: | 1,25 Dihydroxy vitamin D(3) (vitamin D(3)) is an immunomodulator and its deficiency has been associated with susceptibility to tuberculosis. We have studied the immunoregulatory role of vitamin D(3) on various chemokine expression in pulmonary tuberculosis. Peripheral blood mononuclear cells obtained from 21 pulmonary tuberculosis (PTB) patients and 24 healthy controls (HCs) were cultured for 48 h with culture filtrate antigen (CFA) of Mycobacterium tuberculosis with or without vitamin D(3) at a concentration 1 × 10(-7)M. The relative mRNA expression of monocyte chemoattractant protein-1 (MCP-1, CCL2), macrophage inflammatory protein-1α (MIP-1α, CCL3), macrophage inflammatory protein-1β (MIP-1β, CCL4), and regulated upon-activation, normal T cell-expressed and secreted (RANTES, CCL5) and IFN-γ inducible protein-10 (IP-10, CXCL10) chemokines were estimated from 48 h old macrophages using real-time polymerase chain reaction (RT-PCR). The culture supernatants were used to estimate the various chemokines including monokine induced by IFN-γ (MIG, CXCL9) levels using cytometric bead array. In HCs, vitamin D(3) significantly suppressed the MCP-1 mRNA expression of CFA stimulated cells (p=0.0027), while no such effect was observed in PTB patients. Vitamin D(3) showed no significant effect on MIP-1α, MIP-1β and RANTES in both the study groups. The CFA induced IP-10 mRNA and protein expression was significantly suppressed by vitamin D(3) in both the study groups (p0.05). A similar suppressive effect of vitamin D(3) was observed with MIG protein in healthy controls (p=0.0029) and a trend towards a suppression was observed in PTB patients. The suppressive effect of vitamin D(3) is more prominent in CXC chemokines rather than CC chemokines. This suggests that vitamin D(3) may down regulate the recruitment and activation of T-cells through CXC chemokines at the site of infection and may act as a potential anti-inflammatory agent. |
| Databáze: | OpenAIRE |
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