Molecular identification and phylogenetic analysis of Trypanosoma evansi from dromedary camels (Camelus dromedarius) in Egypt, a pilot study
| Autor: | Yasuhiro Fukuda, Noboru Inoue, Chika Tada, Oishi Ryu, Said Amer, Yutaka Nakai |
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| Rok vydání: | 2011 |
| Předmět: |
Trypanosoma
Camelus Sequence analysis Veterinary (miscellaneous) Molecular Sequence Data Population Protozoan Proteins Pilot Projects Biology DNA Ribosomal Polymerase Chain Reaction Mice Trypanosomiasis DNA Ribosomal Spacer Prevalence RNA Ribosomal 18S medicine Animals Cluster Analysis education Ribosomal DNA Phylogeny education.field_of_study Polymorphism Genetic Molecular epidemiology Genes rRNA Sequence Analysis DNA Variable surface glycoprotein DNA Protozoan Ribosomal RNA Trypanosoma evansi biology.organism_classification medicine.disease Virology Molecular biology Animal trypanosomiasis RNA Ribosomal 5.8S Disease Models Animal Infectious Diseases Insect Science Egypt Parasitology RNA Protozoan |
| Zdroj: | Acta Tropica. 117:39-46 |
| ISSN: | 0001-706X |
| Popis: | Animal trypanosomiasis is one of the major constraints of livestock industry in developing countries. In the present study, prevalence of Trypanosome evansi was assessed in the blood of dromedary camels (Camelus dromedarius) brought to Al Bassatein abattoir, Cairo, Egypt, by mouse inoculation test out of 84 tested camels, 4 animals (4.7%) were infected. Molecular analysis was achieved by PCR amplification and sequence analysis of part of ribosomal RNA gene including 18S, ITS1, 5.8S and ITS2 regions. Despite the conserved nature of 18S region, ITS region showed obvious heterogeneity compared to analogous sequences in database. Analysis of transferrin receptor encoding gene (ESAG6) showed variable repertoire in the studied isolates, which may indicate to a novel structure of T. evansi population from Egypt and/or a difference in host range. Furthermore, analysis of variable surface glycoprotein RoTat 1.2 gene marker revealed some heterogeneity at this gene locus. To our knowledge, this is the first molecular analysis of T. evansi in Egypt. |
| Databáze: | OpenAIRE |
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