Ciona intestinalis peroxinectin is a novel component of the peroxidase–cyclooxygenase gene superfamily upregulated by LPS
| Autor: | Daniela Parrinello, Matteo Cammarata, Maria Antonietta Sanfratello, Aiti Vizzini, Valentina Mangano, Nicolò Parrinello |
|---|---|
| Přispěvatelé: | Vizzini,A, Parrinello,D, Sanfratello,MA, Mangano,V, Parrinello,N, Cammarata,M |
| Rok vydání: | 2013 |
| Předmět: |
Lipopolysaccharides
Models Molecular Hemocytes LPS Amino Acid Motifs Molecular Sequence Data Peroxinectin Immunology Integrin Settore BIO/05 - Zoologia Chordate Peroxidase Inflammation Ciona intestinalis Animals Amino Acid Sequence RNA Messenger Cell adhesion Phylogeny Regulation of gene expression Sequence Homology Amino Acid biology Cell adhesion molecule Animal Structures biology.organism_classification Molecular biology Immunity Innate Protein Structure Tertiary Gene Expression Regulation Peroxidases Organ Specificity Myeloperoxidase embryonic structures biology.protein Cell Adhesion Molecules Developmental Biology Endostyle |
| Zdroj: | Developmental & Comparative Immunology. 41:59-67 |
| ISSN: | 0145-305X |
| DOI: | 10.1016/j.dci.2013.03.015 |
| Popis: | Peroxinectins function as hemoperoxidase and cell adhesion factor involved in invertebrate immune reaction. In this study, the ascidian (Ciona intestinalis) peroxinectin gene (CiPxt) and its expression during the inflammatory response have been examined. CiPxt is a new member of the peroxidase-cyclooxygenase gene superfamily that contains both the peroxidase domain and the integrin KGD (Lys-Gly-Asp) binding motif. A phylogenetic tree showed that CiPxt is very close to the chordate group and appears to be the outgroup of mammalian MPO, EPO and TPO clades. The CiPxt molecular structure model resulted superimposable to the human myeloperoxidase. The CiPxt mRNA expression is upregulated by LPS inoculation suggesting it is involved in C. intestinalis inflammatory response. The CiPxt was expressed in hemocytes (compartment/morula cells), vessel epithelium, and unilocular refractile granulocytes populating the inflamed tunic matrix and in the zones 7, 8 and 9 of the endostyle, a special pharynx organs homolog to the vertebrate thyroid gland. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect