Utility of surface enhanced Raman spectroscopy (SERS) for elucidation and simultaneous determination of some penicillins and penicilloic acid using hydroxylamine silver nanoparticles
| Autor: | Marwa R. El-Zahry, Ibrahim H. Refaat, Bernhard Lendl, Erwin Rosenberg, Horria A. Mohamed |
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| Rok vydání: | 2015 |
| Předmět: |
Silver
Inorganic chemistry Substrate (chemistry) Metal Nanoparticles Penicillanic Acid Hydroxylamine Penicillins Surface-enhanced Raman spectroscopy Carbenicillin Spectrum Analysis Raman Silver nanoparticle Analytical Chemistry chemistry.chemical_compound Silver nitrate chemistry medicine Degradation (geology) Penicilloic acid Nuclear chemistry medicine.drug |
| Zdroj: | Talanta. 144 |
| ISSN: | 1873-3573 |
| Popis: | Elucidation and quantitative determination of some of commonly used penicillins (ampicillin, penicillin G and carbenicillin) in the presence of their main degradation product (penicilloic acid) were developed. Forced acidic and basic degradation processes were applied at different time intervals. The formed degradation products were elucidated and quantified using surface enhanced Raman spectroscopy (SERS). Silver nanoparticles (AgNPs) prepared by reduction of silver nitrate using hydroxylamine-HCl in alkaline medium were used as SERS substrate. The results obtained in SERS were confirmed by the application of LC/MS method. The concentration range was 100-600 ng/ml in case of the studied penicillins and 100-700 ng/ml in case of penicilloic acid. An excellent correlation coefficient was found in case of ampicillin (r=0.9993) and in the case of penicilloic acid (r=0.9997). Validation procedures were carried out including precision, robustness and accuracy by comparing F- and t-values of both the proposed and reported methods. |
| Databáze: | OpenAIRE |
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