In vitro responses to purified protein derivate of caprine T lymphocytes following vaccination with live strains of Mycobacterium avium subsp paratuberculosis
| Autor: | Anne K. Storset, G Berntsen, M Valheim, H.J Hasvold |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
T cell
T-Lymphocytes Immunology Paratuberculosis Biology Vaccines Attenuated Microbiology Immunophenotyping Interferon-gamma medicine Animals Interferon gamma Antigens Bacterial Goat Diseases General Veterinary Goats T-cell receptor Receptors Interleukin-2 T lymphocyte medicine.disease Bacterial vaccine Mycobacterium avium subsp. paratuberculosis medicine.anatomical_structure Gene Expression Regulation Bacterial Vaccines CD8 Biomarkers medicine.drug |
| Zdroj: | Veterinary immunology and immunopathology. 90(1-2) |
| ISSN: | 0165-2427 |
| Popis: | Live attenuated vaccines provide protection against intestinal lesions in goats infected with Mycobacterium avium subsp. paratuberculosis. To examine the role of different T lymphocyte subsets in the development of this protective immunity, CD4(+), CD8(+) and gamma delta T cell receptor (TCR)(+) cells from peripheral blood of goat kids vaccinated with live attenuated strains of M. a. paratuberculosis were studied. After in vitro stimulation with purified protein derivate, the expression of gamma-interferon (IFN-gamma) and the activation marker interleukin-2 receptor (IL-2R) was analysed by flow cytometry. A depletion experiment was performed, where the phenotypes and IL-2R expression was studied after stimulation of cultures depleted of a T lymphocyte subpopulation. Close to all of the IFN-gamma producing cells were of the CD4(+) subset, while only a small number were CD8(+) cells. The gamma delta TCR(+) cells were highly activated, but did not produce IFN-gamma after in vitro stimulation. Depletion of CD4(+) cells lead to a decrease in the percentage of total gamma delta TCR(+) cells and gamma delta TCR(+)IL2-R(+) cells. Removing the gamma delta TCR(+) cells increased the relative numbers of CD4(+), but not the CD4(+)IL-2R(+) cells. Insight into the in vitro recall responses of T cell subsets from animals vaccinated with live paratuberculosis vaccines is essential in the development of more efficient vaccines. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect