Case report: live birth following ICSI with non-vital frozen-thawed testicular sperm and oocyte activation with calcium ionophore
| Autor: | Magnus Bach, Anton Neyer, Pierre Vanderzwalmen, Astrid Stecher, Nicolas H. Zech, Martin Zintz |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
Male endocrine system medicine.medical_treatment Oocyte Retrieval chemistry.chemical_element Calcium Intracytoplasmic sperm injection Andrology Human fertilization Pregnancy Genetics medicine Humans Sperm Injections Intracytoplasmic Assisted Reproduction Technologies reproductive and urinary physiology Genetics (clinical) Fertilisation Cryopreservation In vitro fertilisation urogenital system business.industry Infant Newborn Obstetrics and Gynecology Oocyte activation General Medicine Oocyte Spermatozoa Sperm Calcium Ionophores medicine.anatomical_structure Reproductive Medicine chemistry Oocytes Female business Developmental Biology |
| Zdroj: | Journal of Assisted Reproduction and Genetics. 28:411-414 |
| ISSN: | 1573-7330 1058-0468 |
| Popis: | The implementation of intracytoplasmic sperm injection (ICSI) in 1992 gave women whose male partners were diagnosed with severe male factor infertility the chance of having a baby using their partner’s own ejaculated [1] or testicular spermatozoa [2]. Despite reported fertilization rates of approximately 70%, fertilization is dramatically reduced in some cases and does not even occur in 1–3% of ICSI cycles. Most cases of fertilization failure following ICSI can be traced back to a lack of oocyte activation [3], while injection of ejaculated immotile sperm results in either poor or no fertilization [4, 5]. The reason for this lack of oocyte activation may be a deficient cytosolic sperm-associated oocyte activating factor (SAOAF) resulting in a partial or complete inability of the sperm to activate the oocyte [4] or to an inability of the oocyte to decondense the sperm [6]. In 1997, oocyte activation with calcium ionophore was tested in a clinical trial that reported the delivery of a healthy baby [7]. In the same year, oocyte activation with calcium ionophore was performed using injected spermatids [8]. There have been several reports of successful oocyte activation by calcium ionophore treatment in patients with repeated failure of fertilisation using ICSI [9–11] or after ICSI using round-headed spermatozoa [10]. |
| Databáze: | OpenAIRE |
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