Iron regulatory protein 1 is not an early target of cadmium toxicity in mice, but it is sensitive to cadmium stress in a human epithelial cell line

Autor: Jean-Marc Moulis, Estelle Rousselet
Přispěvatelé: Laboratoire de Chimie et Biologie des Métaux (LCBM - UMR 5249), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)
Rok vydání: 2008
Předmět:
inorganic chemicals
Cadmium Poisoning
Iron
MESH: Cadmium
chemistry.chemical_element
Transferrin receptor
Biology
Biochemistry
Intestinal absorption
Cadmium poisoning
MESH: Iron Regulatory Protein 1
Mice
03 medical and health sciences
0302 clinical medicine
MESH: Mice
Inbred C57BL
medicine
Animals
Homeostasis
Humans
MESH: Animals
Iron Regulatory Protein 1
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biochemistry [q-bio.BM]
MESH: Mice
Molecular Biology
030304 developmental biology
MESH: Iron
0303 health sciences
Cadmium
MESH: Humans
Cell Biology
medicine.disease
6. Clean water
Epithelium
Mice
Inbred C57BL
MESH: Hela Cells
MESH: Cadmium Poisoning
medicine.anatomical_structure
chemistry
MESH: Homeostasis
030220 oncology & carcinogenesis
Erythropoiesis
Female
Cellular model
MESH: Female
HeLa Cells
Zdroj: Biochemistry and Cell Biology
Biochemistry and Cell Biology, NRC Research Press, 2008, 86 (5), pp.416-24. ⟨10.1139/o08-120⟩
Biochemistry and Cell Biology, 2008, 86 (5), pp.416-24. ⟨10.1139/o08-120⟩
ISSN: 1208-6002
0829-8211
DOI: 10.1139/o08-120
Popis: International audience; Disruption of iron homeostasis at the levels of intestinal absorption or erythropoiesis contributes to cadmium toxicity. Cellular iron homeostasis in metazoans is maintained by the iron regulatory proteins (IRPs) that regulate the synthesis of proteins involved in the transport, use, and storage of iron. The effect of cadmium intoxication on this regulatory system has been investigated in a cellular model of human epithelium. Cadmium exposure of HeLa cells did not activate the IRPs; rather, the amount of these proteins relative to that of housekeeping proteins decreased. Accordingly, the transferrin receptor mRNA level decreased upon cadmium insult. In a more integrated investigation, separate groups of mice had free access to different doses of cadmium in drinking water for 3 weeks. Cadmium accumulated in all analyzed organs, but its concentration in mouse tissues did not correlate with changes of the activity of the IRPs. The intoxicated mice did not show any sign of anemia, indicating that iron homeostasis was not immediately disrupted after the onset of cadmium accumulation. These data establish that cadmium destabilizes IRPs in mammalian cells, but that iron imbalance is not an early event of cadmium intoxication.
Databáze: OpenAIRE
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