Metabolomics signatures associated with an oral glucose challenge in pregnant women
| Autor: | Courtney Dennis, Kerry A. Pierce, Daniel A. Enquobahrie, Kevin Bullock, Marie Denis, Amy Deik, Bizu Gelaye, Clary B. Clish, Mahlet G. Tadesse, Gloria Larrabure, Michelle A. Williams |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Blood Glucose Endocrinology Diabetes and Metabolism Metabolite pregnant women [EN] Physiology chemistry.chemical_compound 0302 clinical medicine Endocrinology Pregnancy Fatty Acids General Medicine Gestational diabetes S50 - Santé humaine Gestation Female Adult Adolescent 030209 endocrinology & metabolism Article Physiologie humaine Bile Acids and Salts Diglycerides 03 medical and health sciences Young Adult Metabolomics Carnitine Internal Medicine medicine Humans Fatty acid metabolism business.industry Femme nutritional and metabolic diseases Glucose Tolerance Test medicine.disease Omics Lipid Metabolism Diabetes Gestational 030104 developmental biology Glucose chemistry business Body mass index |
| Zdroj: | Diabetes and Metabolism |
| ISSN: | 1878-1780 |
| Popis: | Aim: The oral glucose tolerance test (OGTT), widely used as a gold standard for gestational diabetes mellitus (GDM) diagnosis, provides a broad view of glucose pathophysiology in response to a glucose challenge. We conducted the present study to evaluate metabolite changes before and after an oral glucose challenge in pregnancy; and to examine the extent to which metabolites may serve to predict GDM diagnosis in pregnant women. Methods: Peruvian pregnant women (n = 100) attending prenatal clinics (mean gestation 25 weeks) participated in the study with 23% of them having GDM diagnosis. Serum samples were collected immediately prior to and 2-hours after administration of a 75-g OGTT. Targeted metabolic profiling was performed using a LC-MS based metabolomics platform. Changes in metabolite levels were evaluated using paired Student's t-tests and the change patterns were examined at the level of pathways. Multivariate regression procedures were used to examine metabolite pairwise differences associated with subsequent GDM diagnosis. Results: Of the 306 metabolites detected, the relative concentration of 127 metabolites were statistically significantly increased or decreased 2-hours after the oral glucose load (false discovery rate [FDR] corrected P-value < 0.001). We identified relative decreases in metabolites in acylcarnitines, fatty acids, and diacylglycerols while relative increases were noted among bile acids. In addition, we found that C58:10 triacylglycerol (β = −0.08, SE = 0.04), C58:9 triacylglycerol (β = −0.07, SE = 0.03), adenosine (β = 0.70, SE = 0.32), methionine sulfoxide (β = 0.36, SE = 0.13) were significantly associated with GDM diagnosis even after adjusting for age and body mass index. Conclusions: We identified alterations in maternal serum metabolites, representing distinct cellular and metabolic pathways including fatty acid metabolism, in response to an oral glucose challenge. These findings offer novel perspectives on the pathophysiological mechanisms underlying GDM. |
| Databáze: | OpenAIRE |
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