Regional distribution of 5α-reductase type 2 in the adult rat brain: an immunohistochemical analysis
Autor: | Saturnino Spiga, Marco Bortolato, Angelo Casu, Roberto Frau, M. Paola Castelli, Maria Grazia Ennas, Alberto Casti |
---|---|
Rok vydání: | 2012 |
Předmět: |
Male
Cerebellum Endocrinology Diabetes and Metabolism Hippocampus Biology Article Rats Sprague-Dawley Immunolabeling Endocrinology 3-Oxo-5-alpha-Steroid 4-Dehydrogenase Cortex (anatomy) medicine Animals Protein Isoforms GABAergic Neurons Biological Psychiatry Neurons Endocrine and Autonomic Systems Dentate gyrus Subiculum Brain Entorhinal cortex Immunohistochemistry Olfactory bulb Cell biology Molecular Imaging Rats Psychiatry and Mental health medicine.anatomical_structure nervous system Neuroscience |
Zdroj: | Psychoneuroendocrinology. 38(2) |
ISSN: | 1873-3360 |
Popis: | The enzyme 5α-reductase (5αR) catalyzes the conversion of testosterone and other Δ(4)-3-ketosteroids into their 5α-reduced metabolites. Of the five members of the 5αR family, the type 2 enzyme (5αR2) plays a key role in androgen metabolism, and is abundantly distributed in the urogenital system. Although 5αR2 has been reported to be highly expressed in the brain during early developmental stages, little is currently known on its anatomical and cellular distribution in the adult brain. Thus, the present study was designed to determine the detailed localization of 5αR2 in the adult rat brain, using a highly specific polyclonal antibody against this isoform. Parasagittal and coronal sections revealed 5αR2 immunoreactivity throughout most brain regions, with strong immunolabeling in the layers III and VI of the prefrontal and somatosensory cortex, olfactory bulb, thalamic nuclei, CA3 field of hippocampus, basolateral amygdala and Purkinje cell layer of cerebellum. Lower 5αR2 levels were detected in the hypothalamus and midbrain. Moreover, double labeling fluorescence with confocal laser scanning microscopy (CLSM) revealed that 5αR2 is localized in neurons, but not in glial cells. Specifically, the enzyme was documented in the pyramidal neurons of the cortex by CLSM analysis of simultaneous Golgi-Cox and immunofluorescent staining. Finally, low levels of 5αR2 expression were identified in GABAergic cells across the cortex, hippocampus and striatum. These findings show that, in the adult brain, 5αR2 is distributed in critical regions for behavioral regulation, suggesting that the functional role of this isoform is present throughout the entire lifespan of the individual. |
Databáze: | OpenAIRE |
Externí odkaz: |