Susceptibility of methicillin-resistant Staphylococci clinical isolates to netilmicin and other antibiotics commonly used in ophthalmic therapy
Autor: | Anna Rita Blanco, Andrea Sudano Roccaro, Vincenzo Papa, Carmela Giovanna Spoto |
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Rok vydání: | 2013 |
Předmět: |
Methicillin-Resistant Staphylococcus aureus
medicine.drug_class Antibiotics Moxifloxacin Drug Resistance Levofloxacin Microbial Sensitivity Tests Azithromycin Eye Infections Bacterial Microbiology Cellular and Molecular Neuroscience Staphylococcus epidermidis Vancomycin medicine Tobramycin Humans Netilmicin Etest Aza Compounds biology business.industry SCCmec biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Virology Sensory Systems Anti-Bacterial Agents Ophthalmology Chloramphenicol Quinolines business medicine.drug Fluoroquinolones |
Zdroj: | Current eye research. 38(8) |
ISSN: | 1460-2202 |
Popis: | Purpose: The aim of this study was to test the activity of selected antimicrobial agents commonly used in the treatment of ocular infections against methicillin-resistant Staphylococcus aureus (MRSA) and methicillinresistant Staphylococcus epidermidis (MRSE) isolates. Methods: A total of 43 staphylococci from respiratory tract and ocular infections were characterized for methicillin resistance using the Epsilometer test (E-test), the polymerase chain reaction for mecA gene detection and the PBP2 0 latex agglutination test. A perfect agreement among them was observed in 20 isolates (8 MRSA and 12 MRSE) which were then employed in the susceptibility test by using the agar disk diffusion test (NCCLS). The antibiotics tested were: netilmicin (NET), tobramycin (TOB), azithromycin (AZM), levofloxacin (LEV), moxifloxacin (MXF), chloramphenicol (C) and vancomycin (VA). Results: All MRSE and most (87.5%) of MRSA isolates tested were NET and VA sensitive. The majority of MRSA were found to be resistant to all the other antibiotics, with the exception of C. In particular, 75%, 87% and 100% of the isolates were resistant to fluoroquinolones (LEV and MXF), AZM and TOB, respectively. As for the MRSE group, 25% of the strains tested were resistant to C and MXF while 33%, 42% and 58% of the strains were resistant to LEV, AZM and TOB, respectively. Conclusions: Together with VA, NET was the most effective antibiotic tested against both MRSA and MRSE clinical isolates. The exclusive topical use of NET for the treatment of ocular infections may curtail the emergence, spreading and persistence of antibiotic-resistant bacteria. |
Databáze: | OpenAIRE |
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