METTL14 facilitates global genome repair and suppresses skin tumorigenesis
| Autor: | Yan-Hong Cui, Gayoung Park, Chuan He, Seungwon Yang, Yu-Ying He, Jiangbo Wei, Zizhao Yang, Palak Shah, Xiaolong Cui |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
Skin Neoplasms DNA Repair Carcinogenesis Ultraviolet Rays Nerve Tissue Proteins Biology medicine.disease_cause Methylation Mice Cell Line Tumor medicine Autophagy Animals Humans Genes Tumor Suppressor RNA Messenger Gene knockdown Multidisciplinary Membrane Glycoproteins integumentary system Methyltransferase complex Mutagenesis Intracellular Signaling Peptides and Proteins RNA-Binding Proteins Translation (biology) Methyltransferases Biological Sciences medicine.disease Cell biology DNA-Binding Proteins Female Skin cancer Nucleotide excision repair DNA Damage |
| Zdroj: | Proc Natl Acad Sci U S A |
| ISSN: | 1091-6490 |
| Popis: | Global genome repair (GGR), a subpathway of nucleotide excision repair, corrects bulky helix-distorting DNA lesions across the whole genome and is essential for preventing mutagenesis and skin cancer. Here, we show that METTL14 (methyltransferase-like 14), a critical component of the N(6)-methyladenosine (m(6)A) RNA methyltransferase complex, promotes GGR through regulating m(6)A mRNA methylation–mediated DDB2 translation and suppresses ultraviolet B (UVB) radiation-induced skin tumorigenesis. UVB irradiation down-regulates METTL14 protein through NBR1-dependent selective autophagy. METTL14 knockdown decreases GGR and DDB2 abundance. Conversely, overexpression of wild-type METTL14 but not its enzymatically inactive mutant increases GGR and DDB2 abundance. METTL14 knockdown decreases m(6)A methylation and translation of the DDB2 transcripts. Adding DDB2 reverses the GGR repair defect in METTL14 knockdown cells, indicating that METTL14 facilitates GGR through regulating DDB2 m(6)A methylation and translation. Similarly, knockdown of YTHDF1, an m(6)A reader promoting translation of m(6)A-modified transcripts, decreases DDB2 protein levels. Both METTL14 and YTHDF1 bind to the DDB2 transcript. In mice, skin-specific heterozygous METTL14 deletion increases UVB-induced skin tumorigenesis. Furthermore, METTL14 as well as DDB2 is down-regulated in human and mouse skin tumors and by chronic UVB irradiation in mouse skin, and METTL14 level is associated with the DDB2 level, suggesting a tumor-suppressive role of METTL14 in UVB-associated skin tumorigenesis in association with DDB2 regulation. Taken together, these findings demonstrate that METTL14 is a target for selective autophagy and acts as a critical epitranscriptomic mechanism to regulate GGR and suppress UVB-induced skin tumorigenesis. |
| Databáze: | OpenAIRE |
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