A preliminary study on the immune responses of HPV16-E7 by combined intranasal immunization with lymphotoxin
| Autor: | Ya-Hong Liu, Kuang Wei, Hai-Lin Chen, Bo-Qun Xu, Xiao-Yan Ying |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Papillomavirus E7 Proteins
medicine.medical_treatment Uterine Cervical Neoplasms Immunopotentiator Antibodies Viral Mice Immune system Animals Medicine Antigens Viral Lymphotoxin-alpha Cervical cancer biology business.industry Papillomavirus Infections Immunity HPV infection virus diseases Obstetrics and Gynecology Viral Vaccines Immunotherapy medicine.disease female genital diseases and pregnancy complications Disease Models Animal Lymphotoxin Immunization Immunology biology.protein Female Antibody business |
| Zdroj: | Ginekologia Polska. 91:301-307 |
| ISSN: | 2543-6767 0017-0011 |
| DOI: | 10.5603/gp.2020.0055 |
| Popis: | Objectives: Human papillomavirus (HPV) ranks the first cause of cervical cancer. Cervical cancer has high prevalence rates in women around the world. The HPV-E7 oncoprotein is expressed in cervical cancer and is a target of developing immunotherapies against HPV-associated tumors. However, the antigenicity of this protein is low. Due to this reason, potent adjuvants are required to enhance its therapeutic efficacy. This preliminary study aims to evaluate whether lymphotoxin (LT) could act as an effective immune adjuvant for HPV infection in mice models. Material and methods: Intranasal immunization was used to explore the effect of HPV-E7 and/or LT immune response. After the third intranasal immunization, the titer for the HPV-E7 antibody was detected in serum and vaginal washing fluid. Also, we assessed the expression of chemokine ligand 13 (CXCL13) and Peripheral Node Addressin (PNAd) in the lymph nodes after intranasal immunization with immunohistochemical analysis. Results: compared to HPV-E7 immunization, intranasal immunization with HPV-E7 plus LT significantly increased HPV-E7-specific serum IgG and vaginal IgA titers. Furthermore, the combined use of HPV-E7 and LT strongly induced E7-specific CTL responses. Conclusions: LT can be effective for intranasal immunized HPV-E7 to improve E7-specific immune responses to HPV infection. It is new approach to eradicate chronic HPV infection capable of inducing an effective anti-infection method. |
| Databáze: | OpenAIRE |
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