Re-expression of alpha skeletal actin as a marker for dedifferentiation in cardiac pathologies

Autor: Erik Blaauw, Christine Chaponnier, Wiel Debie, Ronald B. Driesen, Frans C. S. Ramaekers, Fons Verheyen, Jannie Ausma, Richard Cornelussen, M.-H. Lenders, Fawzi A. Babiker, Marcel Borgers
Rok vydání: 2009
Předmět:
Up-Regulation/physiology
Pathology
Atrial Fibrillation/metabolism/pathology
Biological Markers/metabolism
heart failure
Coronary Artery Disease
Actinin
ddc:616.07
030204 cardiovascular system & hematology
Myocytes
Cardiac/metabolism/pathology
0302 clinical medicine
Atrial Fibrillation
Protein Isoforms
Myocyte
Myocytes
Cardiac
hibernation
Cells
Cultured
contractile apparatus
Myocardial Stunning
0303 health sciences
Myocardial Stunning/metabolism/pathology
Goats
Articles
Up-Regulation
medicine.anatomical_structure
Actins/metabolism
Molecular Medicine
Immunohistochemistry
Female
Rabbits
Protein Isoforms/metabolism
Glycogen
Cell Dedifferentiation/physiology
medicine.medical_specialty
Aortic Valve Insufficiency
Down-Regulation
Glycogen/metabolism
Biology
arrhythmia
Aortic Valve Insufficiency/metabolism/pathology
03 medical and health sciences
Coronary Artery Disease/metabolism/pathology
Down-Regulation/physiology
Internal medicine
medicine
Animals
Humans
Actinin/metabolism
Actin
030304 developmental biology
Pressure overload
Myocardial stunning
Cell Biology
myocytes
Cell Dedifferentiation
medicine.disease
Actins
Endocrinology
Ventricle
Heart failure
Biomarkers
Zdroj: Journal of Cellular and Molecular Medicine
Journal of cellular and molecular medicine
Journal of Cellular and Molecular Medicine, Vol. 13, No 5 (2009) pp. 896-908
ISSN: 1582-4934
1582-1838
DOI: 10.1111/j.1582-4934.2008.00523.x
Popis: Differentiation of foetal cardiomyocytes is accompanied by sequential actin isoform expression, i.e. down-regulation of the 'embryonic' alpha smooth muscle actin, followed by an up-regulation of alpha skeletal actin (alphaSKA) and a final predominant expression of alpha cardiac actin (alphaCA). Our objective was to detect whether re-expression of alphaSKA occurred during cardiomyocyte dedifferentiation, a phenomenon that has been observed in different pathologies characterized by myocardial dysfunction. Immunohistochemistry of alphaCA, alphaSKA and cardiotin was performed on left ventricle biopsies from human patients after coronary bypass surgery. Furthermore, actin isoform expression was investigated in left ventricle samples of rabbit hearts suffering from pressure- and volume-overload and in adult rabbit ventricular cardiomyocytes during dedifferentiation in vitro. Atrial goat samples up to 16 weeks of sustained atrial fibrillation (AF) were studied ultrastructurally and were immunostained for alphaCA and alphaSKA. Up-regulation of alphaSKA was observed in human ventricular cardiomyocytes showing down-regulation of alphaCA and cardiotin. A patchy re-expression pattern of alphaSKA was observed in rabbit left ventricular tissue subjected to pressure- and volume-overload. Dedifferentiating cardiomyocytes in vitro revealed a degradation of the contractile apparatus and local re-expression of alphaSKA. Comparable alphaSKA staining patterns were found in several areas of atrial goat tissue during 16 weeks of AF together with a progressive glycogen accumulation at the same time intervals. The expression of alphaSKA in adult dedifferentiating cardiomyocytes, in combination with PAS-positive glycogen and decreased cardiotin expression, offers an additional tool in the evaluation of myocardial dysfunction and indicates major changes in the contractile properties of these cells.
Databáze: OpenAIRE
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