Ultra-high-performance liquid chromatography charge transfer dissociation mass spectrometry (UHPLC-CTD-MS) as a tool for analyzing the structural heterogeneity in carrageenan oligosaccharides
| Autor: | Glen P. Jackson, Praneeth M Mendis, Hélène Rogniaux, Zachary J. Sasiene, David Ropartz |
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| Přispěvatelé: | West Virginia University [Morgantown], Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), INRA Plateforme BIBS, Unité de Recherche Biopolymères, Interactions, Assemblages, Department of Forensic and Investigative Science [Morgantown], the National Science Foundation (NSF) (CHE-1710376) and the National Institute of Health (NIH) : 1R01GM114494-01. |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Capillary action
Dimer Radical ion fragmentation Oligosaccharides Mass spectrometry Carrageenan 01 natural sciences Biochemistry Dissociation (chemistry) Mass Spectrometry Analytical Chemistry Ion 03 medical and health sciences chemistry.chemical_compound Ion-pair reagent [CHIM.ANAL]Chemical Sciences/Analytical chemistry Highly sulfated oligosaccharides Carbohydrate Conformation Chromatography High Pressure Liquid 030304 developmental biology 0303 health sciences Chromatography Charge transfer dissociation Chemistry 010401 analytical chemistry Ion source 0104 chemical sciences High-energy activation Polymerization Rhodophyta |
| Zdroj: | Analytical and Bioanalytical Chemistry Analytical and Bioanalytical Chemistry, Springer Verlag, 2021, pp.Early access. ⟨10.1007/s00216-021-03396-3⟩ |
| ISSN: | 1618-2642 1618-2650 |
| Popis: | International audience; Ultra-high-performance liquid chromatography (UHPLC) with charge transfer dissociation mass spectrometry (CTD-MS) ispresented for the analysis of a mixture of complex sulfated oligosaccharides. The mixture contained kappa (κ), iota (ι), andlambda (λ) carrageenans that contain anhydro bridges, different degrees of sulfation ranging from one to three per dimer,different positioning of the sulfate groups along the backbone, and varying degrees of polymerization (DP) between 4 and 12.Optimization studies using standard mixtures of carrageenans helped establish the optimal conditions for online UHPLC-CTDMS/MS analysis. Optimization included (1) UHPLC conditions; (2) ion source conditions, such as the capillary voltage, dryinggas and nebulizing gas temperature, and flow rate; and (3) CTD-MS conditions, including data-dependent CTD-MS. TheUHPLC-CTD results were contrasted with UHPLC-CID results of the same mixture on the same instrument. Whereas CIDtends to produce B/Y and C/Z ions with many neutral losses, CTD produced more abundant A/X ions and less abundant neutrallosses, which enabled more confident structural detail. The results demonstrate that He-CTD is compatible with the timescale ofUHPLC and provides more structural information about carrageenans compared to state-of-the-art methods like UHPLC-CIDanalysis |
| Databáze: | OpenAIRE |
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