A survey of biofilms on wastewater aeration diffusers suggests bacterial community composition and function vary by substrate type and time
| Autor: | Manel Garrido-Baserba, M. Colby Hunter, Pitiporn Asvapathanagul, Sang Hoon Lee, Hee Deung Park, Peter A. Noble, Diego Rosso, Betty H. Olson |
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| Rok vydání: | 2016 |
| Předmět: |
DNA
Bacterial 0301 basic medicine Time Factors Biofouling Microbial Consortia Polyurethanes 030106 microbiology Silicones Pilot Projects Wastewater Applied Microbiology and Biotechnology Microbiology Diffusion 03 medical and health sciences RNA Ribosomal 16S Surveys and Questionnaires Diffuser (sewage) Principal Component Analysis Bacteria biology Chemistry Biofilm Sequence Analysis DNA General Medicine Ethylenes biology.organism_classification Pulp and paper industry Substrate (marine biology) Oxygen Biodegradation Environmental 030104 developmental biology Elastomers Biofilms Sewage treatment Aeration Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 100:6361-6373 |
| ISSN: | 1432-0614 0175-7598 |
| Popis: | Aeration diffusers in wastewater treatment plants generate air bubbles that promote mixing, distribution of dissolved oxygen, and microbial processing of dissolved and suspended matter in bulk solution. Biofouling of diffusers represents a significant problem to wastewater treatment plants because biofilms decrease oxygen transfer efficiency and increase backpressure on the blower. To better understand biofouling, we conducted a pilot study to survey the bacterial community composition and function of biofilms on different diffuser substrates and compare them to those in the bulk solution. DNA was extracted from the surface of ethylene-propylene-diene monomer (EPDM), polyurethane, and silicone diffusers operated for 15 months in a municipal treatment plant and sampled at 3 and 9 months. The bacterial community composition and function of the biofilms and bulk solution were determined by amplifying the 16S rRNA genes and pyrosequencing the amplicons and raw metagenomic DNA. The ordination plots and dendrograms of the 16S rRNA and functional genes showed that while the bacterial community composition and function of the bulk solution was independent of sampling time, the composition and function of the biofilms differed by diffuser type and testing time. For the EPDM and silicone diffusers, the biofilm communities were more similar in composition to the bulk solution at 3 months than 9 months. In contrast, the bacteria on the polyurethane diffusers were more dissimilar to the bulk solution at 3 months than 9 months. Taken together, the survey showed that the community composition and function of bacterial biofilms depend on the diffuser substrate and testing time, which warrants further elucidation. |
| Databáze: | OpenAIRE |
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